Prolonged Laccase Production by a Cold and pH Tolerant Strain of Penicillium pinophilum MCC 1049 Isolated from a Low Temperature EnvironmentReportar como inadecuado




Prolonged Laccase Production by a Cold and pH Tolerant Strain of Penicillium pinophilum MCC 1049 Isolated from a Low Temperature Environment - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Enzyme Research - Volume 2014 2014, Article ID 120708, 6 pages -

Research Article

Biotechnological Applications, G. B. Pant Institute of Himalayan Environment and Development, Kosi-Katarmal, Almora, Uttarakhand 263 643, India

Department of Biotechnology, Bhimtal Campus, Kumaun University, Nainital, Uttarakhand 263 136, India

Received 25 November 2013; Accepted 29 January 2014; Published 9 March 2014

Academic Editor: Roberto Fernandez Lafuente

Copyright © 2014 Kusum Dhakar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Production of laccase by a cold and pH tolerant strain of Penicillium pinophilum has been investigated under different cultural conditions for up to 35 days of incubation. The fungus was originally isolated from a low temperature environment under mountain ecosystem of Indian Himalaya. The estimations were conducted at 3 temperatures 15, 25, and 35°C, a range of pH 3.5–11.5, and in presence of supplements including carbon and nitrogen sources, vitamins, and antibiotics. Optimum production of laccase was recorded at 25°C optimum temperature for fungal growth and 7.5 pH. The production of enzyme was recorded maximum on day 28  U-L following a slow decline at day 35 of incubation  U-L. Fructose and potassium nitrate 0.2% among nutritional supplements, chloramphenicol 0.1% among antibiotics, and folic acid 0.1% among vitamins were found to be the best enhancers for production of laccase. Relatively lower but consistent production of laccase for a longer period is likely to be an ecologically important phenomenon under low temperature environment. Further, enhancement in production of enzyme using various supplements will be useful for its use in specific biotechnological applications.





Autor: Kusum Dhakar, Rahul Jain, Sushma Tamta, and Anita Pandey

Fuente: https://www.hindawi.com/



DESCARGAR PDF




Documentos relacionados