A Comparative Validation Study of Fluconazole by HPLC and UPLC with Forced Degradation StudyReportar como inadecuado




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Chromatography Research InternationalVolume 2013 2013, Article ID 673150, 5 pages

Research Article

Department of Chemistry, Saurashtra University, Rajkot, Gujarat 360005, India

Banasthali Vidyapith, P.O. BanasthaliVidyapith, Rajasthan 304022, India

Received 18 September 2013; Accepted 27 October 2013

Academic Editor: Toyohide Takeuchi

Copyright © 2013 Hetal Jebaliya et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The simplest stability indicating reversed phase Isocratic HPLC and UPLC methods has been developed and validated for the determination of fluconazole in bulk and solid pharmaceutical dosage form. A SunFire C18 250 × 4.5 mm, 5 μm particle size column has been used for HPLC and BEH C18 100 × 2.1 mm, 1.7 μm particle size column used for UPLC. The Mobile phase consisted of Methanol : Water 70 : 30 for HPLC and Methanol : Water 55 : 45 v-v for UPLC. Isocratic flow was set at 1 mL-min and 0.30 mL-min, respectively, for HPLC and UPLC. For both HPLC and UPLC system detection has been performed at 211 nm with 30°C column oven temperature good elution was obtained at 30°C and injection volume, respectively, 2 μL and 20 μL for HPLC and UPLC.





Autor: Hetal Jebaliya, Madhavi Patel, Yashwant Jadeja, Batuk Dabhi, and Anamik Shah

Fuente: https://www.hindawi.com/



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