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TheScientificWorldJOURNAL - Volume 2 2002, Pages 1132-1137

Short Communication

Faculty of Chemistry, M.V. Lomonosov Moscow State University, Russia

Life Sciences, King’s College London, University of London, UK

Received 12 November 2001; Revised 6 March 2002; Accepted 10 March 2002

Copyright © 2002 Vitalia S. Krikounova et al.


A rapid detection method for the pesticide pentachlorophenol PCP — polarization fluoroimmunoassay PFIA — in the dynamic range of 10–9,000 ppb was developed. PCP may form polychlorinated dibenzo-p-dioxins, making environmental monitoring of this compound an issue of great importance. In order to optimize the PFIA procedure, a number of fluorescein-labeled PCP derivatives and similar compounds tracers were synthesized, and the influence of their structure on PFIA characteristics was studied. Also, two antisera were tested in developing PFIA for PCP. The developed method is highly specific for PCP and can be used for its determination in water samples at a level down to 10 ppb. Total time of the assay for 10 samples is about 7 min. The assay provides a useful and a highly practical screening tool for the processing of large numbers of samples and for the preliminary estimation of potential dioxins contamination in water resources.

Autor: Vitalia S. Krikounova, Ramadan Abuknesha, and Sergei A. Eremin



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