Single Layer Centrifugation with Androcoll-P Can Be Scaled-Up to Process Larger Volumes of Boar SemenReportar como inadecuado




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ISRN Veterinary ScienceVolume 2011 2011, Article ID 548385, 8 pages

Research Article

Division of Reproduction, Department of Clinical Sciences, Swedish University of Agricultural Sciences—SLU, P. O. Box 7054, 75007 Uppsala, Sweden

Faculty of Veterinary Medicine, 3508TD Utrecht, The Netherlands

Institute of Anatomy, Physiology and Biochemistry, Swedish University of Agricultural Sciences, P. O. Box 7011, 75007 Uppsala, Sweden

Quality Genetics, Hörby, Sweden

Received 14 September 2010; Accepted 3 November 2010

Academic Editors: R. Gamble and Y. Yin

Copyright © 2011 Marjet van Wienen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The objective of this study was to scale-up the procedure for Single Layer Centrifugation SLC through Androcoll

-P, as a preliminary step towords processing the whole ejaculate. The first experiment compared Single Layer Centrifugation using 4.5 mL and 15 mL extended ejaculate SLC-4.5 and SLC-15, resp., assessing sperm quality by objective motility analysis, morphology, viability, and the production of reactive oxygen species ROS. In the second experiment, SLC-4.5 was compared to Single Layer Centrifugation with 25 mL extended ejaculate SLC-25 using motility analysis and morphology. In both experiments, normal morphology and linear motility were significantly higher in the SLC-selected samples than in the uncentrifuged controls 𝑃 < . 0 0 1 , whereas total motility and membrane integrity were unchanged. Although ROS production was higher in the SLC-selected samples than in the controls 𝑃 < . 0 1 , this might have been due to the presence of antioxidants in seminal plasma in the latter. In conclusion, there was no difference in sperm quality between SLC-4.5 and SLC-15 samples, or between SLC-4.5 and SLC-25 samples, indicating that the SLC method can be scaled-up successfully.





Autor: Marjet van Wienen, Anders Johannisson, Margareta Wallgren, Joyce Parlevliet, and Jane M. Morrell

Fuente: https://www.hindawi.com/



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