Developmental competence of prepubertal goat oocytes selected with brilliant cresyl blue and matured with cysteamine supplementationReportar como inadecuado




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Abstract : The aim of this study was to assess the effect of oocyte selection using the brilliant cresyl blue BCB test plus the addition of cysteamine to the in vitro maturation IVM medium to improve the in vitro embryo development of prepubertal goat oocytes. The oocytes were exposed to 26 $\mu$M BCB and classified according to their cytoplasm coloration: BCB+ oocytes with blue cytoplasm and BCB- unstained oocytes. The oocytes were matured in a conventional IVM medium supplemented with cysteamine 100 $\mu$M. The control group consisted of oocytes not exposed to BCB and matured without cysteamine. The IVM- oocytes were inseminated and cultured in synthetic oviductal fluid SOF for 7 days. The normal fertilisation rate oocytes showing 2 pronuclei and 1 sperm tail of BCB+ oocytes 40% was higher than those of BCB- 21% and control oocytes 22%. The percentage of morulae plus blastocysts was higher $P$ $



Autor: Elisabeth Rodríguez-González Manel López-Bejar Dolors Izquierdo María-Teresa Paramio

Fuente: https://hal.archives-ouvertes.fr/



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