Osteogenesis from Dental Pulp Derived Stem Cells: A Novel Conditioned Medium Including Melatonin within a Mixture of Hyaluronic, Butyric, and Retinoic AcidsReportar como inadecuado

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Stem Cells International - Volume 2016 2016, Article ID 2056416, 8 pages -

Research Article

Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43-B, 07100 Sassari, Italy

Laboratory of Molecular Biology and Stem Cell Engineering, National Institute of Biostructures and Biosystems, Via Massarenti, 40138 Bologna, Italy

Research Department, Rinaldi Fontani Foundation, Viale Belfiore 43, 50144 Florence, Italy

Department of Biotechnology, University of Natural Resources and Life Sciences Vienna, Muthgasse 18, A-1190 Vienna, Austria

Azienda Ospedaliera Universitaria di Sassari, Via Michele Coppino 26, 07100 Sassari, Italy

Department of Regenerative Medicine, Rinaldi Fontani Institute, Viale Belfiore 43, 50144 Florence, Italy

Stem Wave Institute for Tissue Healing SWITH, Gruppo Villa Maria and Ettore Sansavini Health Science Foundation, Via Provinciale per Cotignola 9, 48022 Lugo, Ravenna, Italy

Received 11 August 2015; Revised 30 November 2015; Accepted 14 December 2015

Academic Editor: Eva Mezey

Copyright © 2016 Margherita Maioli et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Human dental pulp stem cells hDPSCs have shown relevant potential for cell therapy in the orthopedic and odontoiatric fields. The optimization of their osteogenic potential is currently a major challenge. Vascular endothelial growth factor A VEGF A has been recently reported to act as a major conductor of osteogenesis in vitro and in vivo. Here, we attempted to prime endogenous VEGF A expression without the need for viral vector mediated gene transfer technologies. We show that hDPSCs exposure to a mixture of hyaluronic, butyric, and retinoic acids HA + BU + RA induced the transcription of a gene program of osteogenesis and the acquirement of an osteogenic lineage. Such response was also elicited by cell exposure to melatonin, a pleiotropic agent that recently emerged as a remarkable osteogenic inducer. Interestingly, the commitment to the osteogenic fate was synergistically enhanced by the combinatorial exposure to a conditioned medium containing both melatonin and HA + BU + RA. These in vitro results suggest that in vivo osteogenesis might be improved and further studies are needed.

Autor: Margherita Maioli, Valentina Basoli, Sara Santaniello, Sara Cruciani, Alessandro Palmerio Delitala, Roberto Pinna, Egle Mili

Fuente: https://www.hindawi.com/


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