Subchronic Infection of Porphyromonas gingivalis and Tannerella forsythia Stimulates an Immune Response but Not Arthritis in Experimental Murine ModelReport as inadecuate




Subchronic Infection of Porphyromonas gingivalis and Tannerella forsythia Stimulates an Immune Response but Not Arthritis in Experimental Murine Model - Download this document for free, or read online. Document in PDF available to download.

International Journal of Dentistry - Volume 2017 2017, Article ID 2052938, 7 pages - https:-doi.org-10.1155-2017-2052938

Research Article

Universidad Autónoma de Nuevo Leon, Centro de Investigación y Desarrollo en Ciencias de la Salud CIDICS, Facultad de Odontología, Calle Dr. Eduardo Aguirre Pequeño s-n, Colonia Mitras Centro, 64460 Monterrey, NL, Mexico

Universidad Autónoma del Estado de Morelos, Facultad de Farmacia, Avenida Universidad 1001, 62209 Cuernavaca, MOR, Mexico

Universidad Autónoma de Nuevo Leon, Facultad de Medicina Veterinaria y Zootecnia, Centro de Investigación y Desarrollo en Ciencias de la Salud CIDICS, Calle Francisco Villa s-n Colonia Ex-Hacienda el Canada, 66050 Escobedo, NL, Mexico

Universidad de San Martín de Porres, Laboratorio de Investigación en Biología Oral y Molecular, Ciudad Universitaria, Jr. Las Calandrias N° 151-291, Santa Anita, Lima 15009, Peru

Correspondence should be addressed to Myriam A. De La Garza-Ramos

Received 23 February 2017; Revised 8 May 2017; Accepted 10 May 2017; Published 6 June 2017

Academic Editor: Vincent Everts

Copyright © 2017 Jorday Hernández-Aguas et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Studies have proposed that Porphyromonas gingivalis Pg and Tannerella forsythia Tf promote a nonspecific inflammatory response that could produce systemic disease. Oral inoculation of Pg and Tf on the immune and arthritis response was evaluated in BALB-C mice divided into four groups: 1 sham; 2 food contaminated with Pg-Tf; 3 complete Freund’s adjuvant CFA + Pg-Tf; and 4 CFA alone. CFA was administered subcutaneously on days 1 and 14. The arthritis response was monitored for 21 days after day 14 of CFA administration. IL-1β and IL-6 were determined in serum. T cell activation was evaluated by CD25 in salivary lymph nodes or mouse spleen. Pad inflammation appeared by day 19 in the CFA group, but animals with bacteria inoculation presented a delay. A significant increase in IL-6 was found in Groups 3 and 4, but not with respect to IL-1β. We observed an increase in CD25 in cells derived from cervical nodes and in animals with bacteria inoculation and CFA. A local immune response was observed in mice inoculated with Pg and Tf T cell activation; a systemic response was observed with CFA. Since pad inflammation was delayed by bacterial inoculation this suggests that local T cell activation could decrease pad inflammation.





Author: Jorday Hernández-Aguas, José Luis Montiel-Hernández, Myriam A. De La Garza-Ramos, Rosa Velia Ruiz-Ramos, Erandi Escamilla G

Source: https://www.hindawi.com/



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