P2X7 receptors in satellite glial cells mediate high functional expression of P2X3 receptors in immature dorsal root ganglion neuronsReportar como inadecuado




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Molecular Pain

, 8:9

First Online: 07 February 2012Received: 30 June 2011Accepted: 07 February 2012

Abstract

BackgroundThe purinergic P2X3 receptor P2X3R expressed in the dorsal root ganglion DRG sensory neuron and the P2X7 receptor P2X7R expressed in the surrounding satellite glial cell SGC are two major receptors participating in neuron-SGC communication in adult DRGs. Activation of P2X7Rs was found to tonically reduce the expression of P2X3Rs in DRGs, thus inhibiting the abnormal pain behaviors in adult rats. P2X receptors are also actively involved in sensory signaling in developing rodents. However, very little is known about the developmental change of P2X7Rs in DRGs and the interaction between P2X7Rs and P2X3Rs in those animals. We therefore examined the expression of P2X3Rs and P2X7Rs in postnatal rats and determined if P2X7R-P2X3R control exists in developing rats.

FindingsWe immunostained DRGs of immature rats and found that P2X3Rs were expressed only in neurons and P2X7Rs were expressed only in SGCs. Western blot analyses indicated that P2X3R expression decreased while P2X7R expression increased with the age of rats. Electrophysiological studies showed that the number of DRG neurons responding to the stimulation of the P2XR agonist, α,β-meATP, was higher and the amplitudes of α,β-meATP-induced depolarizations were larger in immature DRG neurons. As a result, P2X3R-mediated flinching responses were much more pronounced in immature rats than those found in adult rats. When we reduced P2X7R expression with P2X7R-siRNA in postnatal and adult rats, P2X3R-mediated flinch responses were greatly enhanced in both rat populations.

ConclusionsThese results show that the P2X7R expression increases as rats age. In addition, P2X7Rs in SGCs exert inhibitory control on the P2X3R expression and function in sensory neurons of immature rats, just as observed in adult rats. Regulation of P2X7R expression is likely an effective way to control P2X3R activity and manage pain relief in infants.

KeywordsPurinergic signaling P2X3 receptors P2X7 receptors Dorsal root ganglion P2X7R-P2X3R inhibitory control Neuron-glia interactions Nociception Abnormal pain Postnatal immature rats Development Electronic supplementary materialThe online version of this article doi:10.1186-1744-8069-8-9 contains supplementary material, which is available to authorized users.

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Autor: Yong Chen - Guangwen Li - Li-Yen Mae Huang

Fuente: https://link.springer.com/







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