In Vivo Noninvasive Imaging of Healthy Lower Lip Mucosa: A Correlation Study between High-Definition Optical Coherence Tomography, Reflectance Confocal Microscopy, and HistologyReportar como inadecuado




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Dermatology Research and PracticeVolume 2013 2013, Article ID 205256, 8 pages

Clinical StudyMelanoma Unit, Dermatology Department, Hospital Clínic, C-Villaroel 170, 08036 Barcelona, Spain

Received 4 July 2013; Accepted 28 July 2013

Academic Editor: Iris Zalaudek

Copyright © 2013 Alejandra García-Hernández et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In recent years, technology has allowed the development of new diagnostic techniques which allow real-time, in vivo, noninvasive evaluation of morphological changes in tissue. This study compares and correlates the images and findings obtained by high-definition optical coherence tomography HD-OCT and reflectance confocal microscopy RCM with histology in normal healthy oral mucosa. The healthy lip mucosa of ten adult volunteers was imaged with HD-OCT and RCM. Each volunteer was systematically evaluated by RCM starting in the uppermost part of the epithelium down to the lamina propia. Afterwards, volunteers were examined with a commercially available full-field HD-OCT system using both the “slice” and the “en-face” mode. A “punch” biopsy of the lower lip mucosa was obtained and prepared for conventional histology. The architectural overview offered by “slice” mode HD-OCT correlates with histologic findings at low magnification. In the superficial uppermost layers of the epithelium, RCM imaging provided greater cellular detail than histology. As we deepened into the suprabasal layers, the findings are in accordance with physiological cellular differentiation and correlate with the images obtained from conventional histology. The combined use of these two novel non-invasive imaging techniques provides morphological imaging with sufficient resolution and penetration depth, resulting in quasihistological images.





Autor: Alejandra García-Hernández, Rodrigo Roldán-Marín, Pablo Iglesias-Garcia, and Josep Malvehy

Fuente: https://www.hindawi.com/



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