Genomic Profiling of a Human Leukemic Monocytic Cell-Line THP-1 Exposed to Alpha Particle RadiationReportar como inadecuado

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The Scientific World JournalVolume 2012 2012, Article ID 205038, 8 pages

Research ArticleConsumer and Clinical Radiation Protection Bureau, Health Canada, Healthy Environment and Consumer Safety Branch, Ottawa, ON, Canada K1A 0K9

Received 23 July 2012; Accepted 2 September 2012

Academic Editors: A. Alaiya, Y. Bai, A. S. Balajee, G. Bocci, and V. Krutovskikh

Copyright © 2012 Vinita Chauhan and Matthew Howland. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This study examined alpha α- particle radiation effects on global changes in gene expression in human leukemic monocytic cells THP-1 for the purposes of mining for candidate biomarkers that could be used for the development of a biological assessment tool. THP-1 cells were exposed to α-particle radiation at a dose range of 0 to 1.5 Gy. Twenty-four hours and three days after exposure gene expression was monitored using microarray technology. A total of 16 genes were dose responsive and classified as early onset due to their expression 24 h after exposure. Forty-eight transcripts were dose responsive and classified as late-onset as they were expressed 72 h after exposure. Among these genes, 6 genes were time and dose responsive and validated further using alternate technology. These transcripts were upregulated and associated with biological processes related to immune function, organelle stability and cell signalling-communication. This panel of genes merits further validation to determine if they are strong candidate biomarkers indicative of α-particle exposure.

Autor: Vinita Chauhan and Matthew Howland



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