Phosphorylation of Histone H2A.X in Peripheral Blood Mononuclear Cells May Be a Useful Marker for Monitoring Cardiometabolic Risk in Nondiabetic IndividualsReport as inadecuate




Phosphorylation of Histone H2A.X in Peripheral Blood Mononuclear Cells May Be a Useful Marker for Monitoring Cardiometabolic Risk in Nondiabetic Individuals - Download this document for free, or read online. Document in PDF available to download.

Disease Markers - Volume 2017 2017, Article ID 2050194, 9 pages - https:-doi.org-10.1155-2017-2050194

Research Article

Department of Food Science and Nutrition, Brain Busan 21 Project, Dong-A University, Busan 49315, Republic of Korea

Department of Biomedical Sciences, Center for Creative Biomedical Scientists, Chonnam National University, Gwangju 61469, Republic of Korea

Department of Rehabilitation Medicine, Dong-A University Hospital, Busan 49315, Republic of Korea

Human Life Research Center, Dong-A University, Busan 49315, Republic of Korea

Correspondence should be addressed to Oh Yoen Kim

Received 1 February 2017; Revised 2 April 2017; Accepted 11 April 2017; Published 9 May 2017

Academic Editor: Shih-Ping Hsu

Copyright © 2017 So Ra Yoon et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Phosphorylation of H2A.X serine 139 in the histone H2A family located in the downstream of the DNA damage kinase signaling cascade is an important indicator of DNA damage. Recently, phosphorylation of H2A.X was proposed as a sensitive biomarker of aging. This study investigated if phosphorylation of H2A.X in peripheral blood mononuclear cells PBMCs is associated with cardiometabolic risk in nondiabetic individuals. Basic parameters and oxidative stress-inflammatory markers were measured in nondiabetic healthy Koreans . Phosphorylation of H2A.X was measured randomly among the study subjects using a flow cytometer. According to the number of metabolic syndrome risk factor MetS-RF, the study subjects were subdivided into -super healthy- , and -MetS-risk- , groups. Phosphorylation of H2A.X in PBMCs percentages and mean fluorescence intensity was significantly higher in the MetS-risk group than in the super healthy group after adjusting for age, sex, cigarette smoking, and alcohol consumption. Phosphorylated H2A.X was positively correlated with the number of MetS-RF as well as waist circumference, blood pressures, triglyceride, HbA1C, oxidized LDL, high sensitivity C-reactive protein, tumor necrosis factor-alpha, and alanine aminotransferase after the adjustment. The present study suggested that phosphorylated H2A.X in circulating PBMCs measured by flow cytometer may be a useful marker for monitoring cardiometabolic risk in nondiabetic individuals.





Author: So Ra Yoon, Juhyun Song, Jong Hwa Lee, and Oh Yoen Kim

Source: https://www.hindawi.com/



DOWNLOAD PDF




Related documents