Local Gene Delivery System by Bubble Liposomes and Ultrasound Exposure into Joint SynoviumReport as inadecuate




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Journal of Drug DeliveryVolume 2011 2011, Article ID 203986, 7 pages

Research Article

Department of Drug and Gene Delivery Systems, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan

Department of Biopharmaceutics, Teikyo University, 1091-1 Suwarashi, Midori-ku, Sagamihara, Kanagawa 252-5195, Japan

Department of Periodontology, Showa University School of Dentistry, 2-1-1 Kitasenzoku, Ohta-ku, Tokyo 145-8515, Japan

Received 17 January 2011; Accepted 1 March 2011

Academic Editor: Susan Hua

Copyright © 2011 Yoichi Negishi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Recently, we have developed novel polyethylene glycol modified liposomes bubble liposomes; BL entrapping an ultrasound US imaging gas, which can work as a gene delivery tool with US exposure. In this study, we investigated the usefulness of US-mediated gene transfer systems with BL into synoviocytes in vitro and joint synovium in vivo. Highly efficient gene transfer could be achieved in the cultured primary synoviocytes transfected with the combination of BL and US exposure, compared to treatment with plasmid DNA pDNA alone, pDNA plus BL, or pDNA plus US. When BL was injected into the knee joints of mice, and US exposure was applied transcutaneously to the injection site, highly efficient gene expression could be observed in the knee joint transfected with the combination of BL and US exposure, compared to treatment with pDNA alone, pDNA plus BL, or pDNA plus US. The localized and prolonged gene expression was also shown by an in vivo luciferase imaging system. Thus, this local gene delivery system into joint synovium using the combination of BL and US exposure may be an effective means for gene therapy in joint disorders.





Author: Yoichi Negishi, Yuka Tsunoda, Yoko Endo-Takahashi, Yusuke Oda, Ryo Suzuki, Kazuo Maruyama, Matsuo Yamamoto, and Yukihiko Ar

Source: https://www.hindawi.com/



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