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Journal of Nanomaterials - Volume 2016 2016, Article ID 2036029, 11 pages -

Research Article

Department of Bioengineering, University of Louisville, Louisville, KY 40208, USA

Department of Medicine and James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA

Received 22 January 2016; Revised 24 March 2016; Accepted 29 March 2016

Academic Editor: Gang Liu

Copyright © 2016 Tyler Brann et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The selective exposure of cancerous tissue to systemically delivered chemotherapeutic agents remains a major challenge facing cancer therapy. To address this question, a near infrared responsive oligonucleotide-coated AS1411, hairpin, or both gold nanoplate loaded with doxorubicin is demonstrated to be nontoxic to cells without triggered release, while being acutely toxic to cells after 5 minutes of laser exposure to trigger DOX release. Conjugation of oligonucleotides to the nanoplates is confirmed by an average increase in hydrodynamic diameter of 30.6 nm, an average blue shift of the plasmon resonance peak by 36 nm, and an average −10 mV shift in zeta potential of the particles. DOX loading through intercalation into the hairpin DNA structure is confirmed through fluorescence measurements. For both GNP-Hairpin and GNP-Hairpin-AS1411, ~60% of loaded DOX is released after the first 5 minutes of laser exposure  nm, with complete release after two more 5-minute exposures. Preliminary proof of concept is demonstrated in vitro using A549 and MDA-MB-231 cell lines as models for breast and lung cancer, respectively. Exposure of cells to untriggered DOX-loaded conjugate with no laser exposure results in little to no toxicity, while laser-triggered release of DOX causes significant cell death.





Autor: Tyler Brann, Dhruvinkumar Patel, Rajat Chauhan, Kurtis T. James, Paula J. Bates, Mohammad Tariq Malik, Robert S. Keynton, a

Fuente: https://www.hindawi.com/



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