Regulation of Wnt signaling by nociceptive input in animal modelsReportar como inadecuado

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Molecular Pain

, 8:47

First Online: 19 June 2012Received: 10 January 2012Accepted: 04 June 2012


BackgroundCentral sensitization-associated synaptic plasticity in the spinal cord dorsal horn SCDH critically contributes to the development of chronic pain, but understanding of the underlying molecular pathways is still incomplete. Emerging evidence suggests that Wnt signaling plays a crucial role in regulation of synaptic plasticity. Little is known about the potential function of the Wnt signaling cascades in chronic pain development.

ResultsFluorescent immunostaining results indicate that β-catenin, an essential protein in the canonical Wnt signaling pathway, is expressed in the superficial layers of the mouse SCDH with enrichment at synapses in lamina II. In addition, Wnt3a, a prototypic Wnt ligand that activates the canonical pathway, is also enriched in the superficial layers. Immunoblotting analysis indicates that both Wnt3a a β-catenin are up-regulated in the SCDH of various mouse pain models created by hind-paw injection of capsaicin, intrathecal i.t. injection of HIV-gp120 protein or spinal nerve ligation SNL. Furthermore, Wnt5a, a prototypic Wnt ligand for non-canonical pathways, and its receptor Ror2 are also up-regulated in the SCDH of these models.

ConclusionOur results suggest that Wnt signaling pathways are regulated by nociceptive input. The activation of Wnt signaling may regulate the expression of spinal central sensitization during the development of acute and chronic pain.

KeywordsWnt Synapse Spinal dorsal horn Pain Nociception β-catenin Electronic supplementary materialThe online version of this article doi:10.1186-1744-8069-8-47 contains supplementary material, which is available to authorized users.

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Autor: Yuqiang Shi - Subo Yuan - Bei Li - Jigong Wang - Susan M Carlton - Kyungsoon Chung - Jin Mo Chung - Shao-Jun Tang


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