TRPV1, but not TRPA1, in primary sensory neurons contributes to cutaneous incision-mediated hypersensitivityReportar como inadecuado




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Molecular Pain

, 9:9

First Online: 04 March 2013Received: 24 September 2012Accepted: 21 February 2013

Abstract

BackgroundMechanisms underlying postoperative pain remain poorly understood.
In rodents, skin-only incisions induce mechanical and heat hypersensitivity similar to levels observed with skin plus deep incisions.
Therefore, cutaneous injury might drive the majority of postoperative pain.
TRPA1 and TRPV1 channels are known to mediate inflammatory and nerve injury pain, making them key targets for pain therapeutics.
These channels are also expressed extensively in cutaneous nerve fibers.
Therefore, we investigated whether TRPA1 and TRPV1 contribute to mechanical and heat hypersensitivity following skin-only surgical incision.

ResultsBehavioral responses to mechanical and heat stimulation were compared between skin-incised and uninjured, sham control groups.
Elevated mechanical responsiveness occurred 1 day post skin-incision regardless of genetic ablation or pharmacological inhibition of TRPA1.
To determine whether functional changes in TRPA1 occur at the level of sensory neuron somata, we evaluated cytoplasmic calcium changes in sensory neurons isolated from ipsilateral lumbar 3–5 DRGs of skin-only incised and sham wild type WT mice during stimulation with the TRPA1 agonist cinnamaldehyde.
There were no changes in the percentage of neurons responding to cinnamaldehyde or in their response amplitudes.
Likewise, the subpopulation of DRG somata retrogradely labeled specifically from the incised region of the plantar hind paw showed no functional up-regulation of TRPA1 after skin-only incision.
Next, we conducted behavior tests for heat sensitivity and found that heat hypersensitivity peaked at day 1 post skin-only incision.
Skin incision-induced heat hypersensitivity was significantly decreased in TRPV1-deficient mice.
In addition, we conducted calcium imaging with the TRPV1 agonist capsaicin.
DRG neurons from WT mice exhibited sensitization to TRPV1 activation, as more neurons 66% from skin-incised mice responded to capsaicin compared to controls 46%, and the sensitization occurred specifically in isolectin B4 IB4-positive neurons where 80% of incised neurons responded to capsaicin compared to just 44% of controls.

ConclusionsOur data suggest that enhanced TRPA1 function does not mediate the mechanical hypersensitivity that follows skin-only surgical incision.
However, the heat hypersensitivity is dependent on TRPV1, and functional up-regulation of TRPV1 in IB4-binding DRG neurons may mediate the heat hypersensitivity after skin incision injury.

KeywordsTRPV1 TRPA1 Postoperative pain Sensory neuron Keratinocyte Mechanical Heat Skin Cutaneous Calcium imaging AbbreviationsTRPV1Transient receptor potential vanilloid 1

TRPA1Transient receptor potential ankyrin 1

DRGDorsal root ganglion

WTWild type

IB4Isolectin B4

TRPA1 KOTransient receptor potential ankyrin 1 knockout

TRPV1 KOTransient receptor potential vanilloid 1 knockout

PODPostoperative day

CINNCinnamaldehyde

WGAWheat germ agglutinin

CAPCapsaicin.

Electronic supplementary materialThe online version of this article doi:10.1186-1744-8069-9-9 contains supplementary material, which is available to authorized users.

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Autor: Marie E Barabas - Cheryl L Stucky

Fuente: https://link.springer.com/



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