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International Journal of Colorectal Disease

, Volume 27, Issue 11, pp 1445–1453

First Online: 06 May 2012Accepted: 13 April 2012

Abstract

PurposeAn herbal preparation, STW 5, used clinically in functional dyspepsia and irritable bowel syndrome, has been shown to possess properties that may render it useful in inflammatory bowel disease IBD. The present work was conducted to study its effectiveness in a rat model of IBD.

MethodsAn experimental model reflecting ulcerative colitis in man was adopted, whereby colitis was induced in Wistar rats by feeding them 5 % dextran sulfate sodium DSS in drinking water for one week. STW 5 and sulfasalazine as a reference standard were administered orally daily for 1 week before colitis induction and continued during DSS feeding. The animals were then sacrificed, and the severity of colitis was evaluated macroscopically and microscopically. Colon samples were homogenized for determination of reduced glutathione, tumor necrosis factor-α, and cytokine-induced neutrophil chemoattractant-3 as well as myeloperoxidase, glutathione peroxidase, and superoxide dismutase. In addition, colon segments were suspended in an organ bath to test their reactivity towards carbachol, KCl, and trypsin.

ResultsSTW 5 and sulfasalazine were both effective in preventing the shortening of colon length and the increase in both colon mass index and total histology score as well as the changes in biochemical parameters measured except changes in dismutase activity. DSS-induced colitis led to marked depression in colonic responsiveness to the agents tested ex vivo, an effect which was normalized by both drugs.

ConclusionsThe findings point to a potential usefulness of STW 5 in the clinical setting of ulcerative colitis.

KeywordsDSS colitis Colonic responsiveness STW 5 Cytokines  Download fulltext PDF



Autor: Walaa Wadie - Heba Abdel-Aziz - Hala F. Zaki - Olaf Kelber - Dieter Weiser - Mohamed T. Khayyal

Fuente: https://link.springer.com/article/10.1007/s00384-012-1473-z



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