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Clinical Orthopaedics and Related Research®

, Volume 472, Issue 5, pp 1357–1364

First Online: 13 December 2013Received: 19 September 2013Accepted: 27 November 2013

Abstract

BackgroundAlthough relatively uncommon, spontaneous healing from a meniscus injury has been observed even within the avascular area.
This may be the result of the existence of mesenchymal stem cells in synovial fluid.

Questions-purposesThe purpose of this study was to investigate whether mesenchymal stem cells existed in the synovial fluid of the knee after meniscus injury.

MethodsSynovial fluid was obtained from the knees of 22 patients with meniscus injury just before meniscus surgery and from 8 volunteers who had no history of knee injury.
The cellular fraction of the synovial fluid was cultured for 14 days followed by analysis for multilineage potential and presentation of surface antigens characteristic of mesenchymal stem cells.
Colony-forming efficiency and proliferation potential were also compared between the two groups.

ResultsCells with characteristics of mesenchymal stem cells were observed in the synovial fluid of injured knees to a much greater degree than in uninjured knees.
The colony-forming cells derived from the synovial fluid of the knee with meniscus injury had multipotentiality and surface epitopes identical to mesenchymal stem cells.
The average number of colony formation, obtained from 1 mL of synovial fluid, in meniscus-injured knees was 250, higher than that from healthy volunteers, which was 0.5 p < 0.001.
Total colony number per synovial fluid volume was positively correlated with the postinjury period r = 0.77, p < 0.001.

ConclusionsMesenchymal stem cells were found to exist in synovial fluid from knees after meniscus injury.
Mesenchymal stem cells were present in higher numbers in synovial fluid with meniscus injury than in normal knees.
Total colony number per synovial fluid volume was positively correlated with the postinjury period.

Clinical RelevanceOur current human study and previous animal studies suggest the possibility that mesenchymal stem cells in synovial fluid increase after meniscus injury contributing to spontaneous meniscus healing.

One of the authors IS was supported by the Project for Realization of Regenerative Medicine by the Ministry of Education, Culture, Sports, Science and Technology MEXT, Japan.

All ICMJE Conflict of Interest Forms for authors and Clinical Orthopaedics and Related Research editors and board members are on file with the publication and can be viewed on request.

Each author certifies that his institution approved the human protocol for this investigation that all investigations were conducted in conformity with ethical principles of research, and that informed consent for participation in the study was obtained.

A comment to this article is available at http:-dx.doi.org-10.1007-s11999-014-3562-5.

An erratum to this article can be found at http:-dx.doi.org-10.1007-s11999-013-3443-3.

A comment to this article is available at http:-dx.doi.org-10.1007-s11999-013-3433-5.

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Autor: Yu Matsukura - Takeshi Muneta - Kunikazu Tsuji - Hideyuki Koga - Ichiro Sekiya

Fuente: https://link.springer.com/article/10.1007/s11999-013-3418-4



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