Uptake of the antisecretory factor peptide AF-16 in rat blood and cerebrospinal fluid and effects on elevated intracranial pressureReportar como inadecuado




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Acta Neurochirurgica

, Volume 157, Issue 1, pp 129–137

First Online: 24 September 2014Received: 08 June 2014Accepted: 01 September 2014

Abstract

BackgroundAF-16 is a 16-amino-acid-long peptide derived from the amino-terminal part of the endogenous protein, antisecretory factor AF.
AF-16 in vivo has been shown to regulate dysfunctions in the water and ion transport system under various pathological conditions and also to counteract experimentally increased tissue pressure.

MethodsRats were subjected to a cryogenic brain injury in order to increase the intracranial pressure ICP.
The distribution of AF-16 in blood and CSF after intravenous or intranasal administration was determined in injured and control rats.
ICP was monitored in freely moving, awake rats, by means of an epidural pressure transducer catheter connected to a wireless device placed subcutaneously on the skull.
The continuous ICP registrations were achieved by means of telemetry.

ResultsIntranasal administration of AF-16 resulted in a significantly higher CSF concentrations of AF-16 in injured than in control rats, 1.3 versus 0.6 ng-ml, whereas no difference between injured and control rats was seen when AF-16 was given intravenously.
Rats subjected to cryogenic brain injury developed gradually increasing ICP levels.
Intranasal administration of AF-16 suppressed the increased ICP to normal values within 30 min.

ConclusionOptimal AF-16 concentrations in CSF are achieved after intranasal administration in rats subjected to a cryogenic brain injury.
The ability of AF-16 to suppress an increased ICP was manifested.

KeywordsAF-16 uptake Intranasal administration Brain oedema Intracranial pressure Telemetry Continuous recording Rat  Download fulltext PDF



Autor: Mohamed Al-Olama - Stefan Lange - Ivar Lönnroth - Kliment Gatzinsky - Eva Jennische

Fuente: https://link.springer.com/



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