Developmental Regulation of Sialoadhesin Sheep Erythrocyte Receptor, a Macrophage-Cell Interaction Molecule Expressed in Lymphohemopoietic TissuesReportar como inadecuado




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Developmental Immunology - Volume 2 1992, Issue 1, Pages 7-17



Walter and Eliza Hall Institute, Melbourne, Australia

Pasteur Institute, Paris, France

Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, United Kingdom



Copyright © 1992 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Stromal macrophages in lymphohemopoietic tissues express novel macrophagerestrictedplasma membrane receptors involved in nonphagocytic interactions withother hemopoietic cells. One such receptor with lectinlike specificity for sialylatedglycoconjugates on sheep erythrocytes and murine hemopoietic cells has beencharacterized immunochemically and termed sialoadhesin. We have examinedsialoadhesin expression during mouse development to learn more about its regulationand function. Immunocytochemical, rosetting, and Western blot studies show thatsialoadhesin is first detected on fetal liver macrophages on day 18 of development, 7days after numerous F4-80

macrophages are found within erythroblastic islands. Inspleen and bone marrow, sialoadhesin appears between day 18 and birth, in parallelwith myeloid development. Strongly labeled macrophages in the marginal zone ofspleen, characteristic of adult lymphoid tissues, appeared gradually between 1–4Isolation of fetal liver macrophages at day 14 confirmed that sialoadhesin was notinvolved in the binding of erythroblasts, which is mediated by a distinct cationdependentreceptor Morris et al., 1988, p. 649. Sialoadhesin could be expressed byisolated fetal liver macrophages after cultivation in adult mouse serum, a known sourceof inducer activity, but was not dependent on the presence of this inducer, unlike adultderivedmadrophages. Fetal plasma contained inducing activity on day 13, but adultlevels were not reached until 2 weeks postnatally. These studies show that sialoadhesinis differentially regulated compared with the erythroblast receptor and F4-80 antigen,that it is not required for fetal erythropoiesis, and that its induction on stromalmacrophages is delayed until the onset of myeloid and lymphoid development.Sialoadhesin provides a marker to study maturation and functions of macrophagesduring ontogeny of the lymphohemopoietic system.





Autor: Lynn Morris, Paul R. Crocker, Maxine Hill, and Siamon Gordon

Fuente: https://www.hindawi.com/



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