Methane-rich saline attenuates ischemia-reperfusion injury of abdominal skin flaps in rats via regulating apoptosis levelReportar como inadecuado




Methane-rich saline attenuates ischemia-reperfusion injury of abdominal skin flaps in rats via regulating apoptosis level - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

BMC Surgery

, 15:92

First Online: 31 July 2015Received: 28 March 2015Accepted: 16 July 2015

Abstract

BackgroundIn plastic surgery, skin damage induced by ischemia-reperfusion I-R is a multifactorial process that often occurs. Methane gas has been reported to be a new therapeutic gas for attenuating I-R injury. In this study, we assessed the effects of methane-rich saline MRS in regulating apoptosis on skin flap I-R injury.

MethodsMale Sprague–Dawley rats, 6–8 weeks old, were divided randomly into three groups: one sham surgery group SH and two surgery groups. After undergoing 6 h of I-R management of an abdominal skin flap, surgery groups were treated with physiological saline I-R-P or methane-rich saline I-R-M. On the 3rd postoperative day, a laser Doppler flowmeter was used to measure flap blood supply, and hematoxylin and eosin HandE staining was used to observe morphological changes. TdT-mediated dUTP-X nick end labeling TUNEL staining was also used to observe early apoptosis and is presented as the percentage of TUNEL-positive cells. Moreover, pASK-1, pJNK, Bcl-2 and Bax were detected by immunohistochemical technology. Caspase-3 activity was also measured to evaluate the effects of MRS.

ResultsCompared to the I-R-P group, the flaps in the I-R-M group presented a larger survival area and better blood perfusion with less inflammatory infiltration and cell apoptosis, a higher expression of Bcl-2, a lower expression of pASK-1, pJNK and Bax, and a lower caspase-3 activity.

ConclusionAccording to the results, MRS attenuated I-R injury by regulating apoptosis and has the potential to be applied as a new therapy for improving skin flap survival.

KeywordsMethane-rich saline Ischemia-reperfusion injury Skin flaps Apoptosis Kexin Song and Mingzi Zhang first Author

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Autor: Kexin Song - Mingzi Zhang - Jianqiang Hu - Yunqi Liu - Yifang Liu - Youbin Wang - Xuemei Ma

Fuente: https://link.springer.com/article/10.1186/s12893-015-0075-4



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