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Evidence-Based Complementary and Alternative Medicine - Volume 2017 2017, Article ID 2170258, 8 pages - https:-doi.org-10.1155-2017-2170258

Research ArticleSchool of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China

Correspondence should be addressed to Xiaobo Li

Received 5 February 2017; Revised 3 May 2017; Accepted 7 June 2017; Published 16 July 2017

Academic Editor: Letizia Angiolella

Copyright © 2017 Yunfei Ji et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Sijunzi Decoction SJZD is a conventional prescription for curing spleen deficiency in Traditional Chinese Medicine and polysaccharide is its main ingredient. In order to explore the effective ingredients contributing to the immunological activity of SJZD, we isolated and purified seven homogeneous polysaccharides from Radix Ginseng RS-3-1 and RS-3-2, Rhizoma Atractylodis Macrocephalae BZ-3-1, BZ-3-2, and BZ-3-3, Poria FL-3-1, and Radix Glycyrrhizae GC-3-1 decoctions, respectively. The molecular weight of seven homogeneous polysaccharides ranged from 5.42 × 10

to 5.65 × 10

 Da. Monosaccharide composition determined by GC-MS analysis showed that these polysaccharides were primarily composed of Rha, Ara, Xyl, Man, Glc, and Gal with various ratios. Immunological activity assay revealed that polysaccharides from four crude drug components of SJZD displayed inhibitory effects on the complement system. RS-3-1, BZ-3-1, FL-3-1, and GC-3-1 could significantly enhance the phagocytosis and increase the NO production and tumor necrosis factor TNF-α level in RAW 264.7 cells . These results demonstrated the immunological activities of these polysaccharides from the four crude drugs. This study supports the therapeutic effect of SJZD in clinical use and is essential for further identification the immunopolysaccharide from SJZD decoction.





Autor: Yunfei Ji, Ruijun Wang, Ying Peng, Chongsheng Peng, and Xiaobo Li

Fuente: https://www.hindawi.com/



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