Nautilia abyssi sp. nov., a thermophilic, chemolithoautotrophic, sulfur-reducing bacterium isolated from an East Pacific Rise hydrothermal ventReportar como inadecuado




Nautilia abyssi sp. nov., a thermophilic, chemolithoautotrophic, sulfur-reducing bacterium isolated from an East Pacific Rise hydrothermal vent - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

* Corresponding author 1 LM2E - Laboratoire de microbiologie des environnements extrêmophiles 2 LEP - Laboratoire Environnement Profond

Abstract : A novel strictly anaerobic, thermophilic, sulfur-reducing bacterium, designated PH1209T, was isolated from an East Pacific Rise hydrothermal vent 1 degrees N sample and studied using a polyphasic taxonomic approach. Cells were Gram-negative, motile rods approx. 1.60 x 0.40 microm with a single polar flagellum. Strain PH1209T grew at temperatures between 33 and 65 degrees C optimum 60 degrees C, from pH 5.0 to 8.0 optimum 6.0-6.5, and between 2 and 4 % w-v NaCl optimum 3 %. Cells grew chemolithoautotrophically with H2 as an energy source, S0 as an electron acceptor and CO2 as a carbon source. Strain PH1209T was also able to use peptone and yeast extract as carbon sources. The G+C content of the genomic DNA was 35 mol%. Phylogenetic analyses based on 16S rRNA gene sequencing showed that strain PH1209T fell within the order Nautiliales, in the class Epsilonproteobacteria. Comparative 16S rRNA gene sequence analysis indicated that strain PH1209T belonged to the genus Nautilia and shared 97.2 and 98.7 % 16S rRNA gene sequence identity, respectively, with the type strains of Nautilia lithotrophica and Nautilia profundicola. It is proposed, from the polyphasic evidence, that the strain represents a novel species, Nautilia abyssi sp. nov.; the type strain is PH1209T =DSM 21157T=JCM 15390T.

Keywords : East Pacific Rise Bacteria Hydrothermal vent Marine environment Sulfur Thermophily Taxonomy New species





Autor: Karine Alain - Nolwenn Callac - Marianne Guégan - Françoise Lesongeur - Philippe Crassous - Marie-Anne Cambon-Bonavita - Joël

Fuente: https://hal.archives-ouvertes.fr/



DESCARGAR PDF




Documentos relacionados