Levels of gastrin-releasing peptide and substance P in synovial fluid and serum correlate with levels of cytokines in rheumatoid arthritisReportar como inadecuado

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Arthritis Research and Therapy

, 7:R416

First Online: 07 February 2005Received: 27 September 2004Revised: 21 December 2004Accepted: 04 January 2005


It is well known that cytokines are highly involved in the disease process of rheumatoid arthritis RA. Recently, targeting of neuropeptides has been suggested to have potential therapeutic effects in RA. The aim of this study was to investigate possible interrelations between five neuropeptides bombesin-gastrin-releasing peptide BN-GRP, substance P SP, vasoactive intestinal peptide, calcitonin-gene-related peptide, and neuropeptide Y and the three cytokines tumour necrosis factor TNF-α, IL-6, and monocyte chemoattractant protein-1 in synovial fluid of patients with RA. We also investigated possible interrelations between these neuropeptides and soluble TNF receptor 1 in serum from RA patients. Synovial fluid and sera were collected and assayed with ELISA or RIA. The most interesting findings were correlations between BN-GRP and SP and the cytokines. Thus, in synovial fluid, the concentrations of BN-GRP and SP grouped together with IL-6, and SP also grouped together with TNF-α and monocyte chemoattractant protein-1. BN-GRP and SP concentrations in synovial fluid also grouped together with the erythrocyte sedimentation rate. In the sera, BN-GRP concentrations and soluble TNF receptor 1 concentrations were correlated. These results are of interest because blocking of SP effects has long been discussed in relation to RA treatment and because BN-GRP is known to have trophic and growth-promoting effects and to play a role in inflammation and wound healing. Furthermore, the observations strengthen a suggestion that combination treatment with agents interfering with neuropeptides and cytokines would be efficacious in the treatment of RA. In conclusion, BN-GRP and SP are involved together with cytokines in the neuroimmunomodulation that occurs in the arthritic joint.

Keywordscytokines gastrin-releasing peptide rheumatoid arthritis substance P TNF-α AbbreviationsBN-GRPbombesin-gastrin-releasing peptide

CGRPcalcitonin-gene-related peptide

ELISAenzyme-linked immunosorbent assay

ESRerythrocyte sedimentation rate

GRPgastrin-releasing peptide


MCP-1monocyte chemoattractant protein-1

NPYneuropeptide Y

RArheumatoid arthritis


SPsubstance P

sTNFR1soluble TNF receptor 1

ThT helper cells

TNFtumour necrosis factor

VIPvasoactive intestinal peptide.

Electronic supplementary materialThe online version of this article doi:10.1186-ar1503 contains supplementary material, which is available to authorized users.

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Autor: Ola Grimsholm - Solbritt Rantapää-Dahlqvist - Sture Forsgren

Fuente: https://link.springer.com/

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