Diagnostic value of anti-human citrullinated fibrinogen ELISA and comparison with four other anti-citrullinated protein assaysReportar como inadecuado

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Arthritis Research and Therapy

, 8:R122

First Online: 19 July 2006Received: 28 March 2006Revised: 06 July 2006Accepted: 13 July 2006


We studied the diagnostic performance of the anti-human citrullinated fibrinogen antibody AhFibA ELISA for rheumatoid arthritis RA in a consecutive cohort population 1 and evaluated the agreement between the AhFibA ELISA and four other assays for anti-citrullinated protein-peptide antibodies ACPAs as well as rheumatoid factor in patients with longstanding RA population 2. Population 1 consisted of 1024 patients with rheumatic symptoms; serum samples from these patients were sent to our laboratory for ACPA testing within the context of a diagnostic investigation for RA. Ninety-two of these patients were classified as having RA according to the American College of Rheumatology criteria and 463 were classified as non-RA patients. Population 2 consisted of 180 patients with longstanding RA and was used to assess agreement and correlations between five ACPA assays: anti-cyclic citrullinated peptide CCP1 and anti-CCP2 antibodies were detected using a commercially available ELISA, AhFibA using ELISA, and anti-PepA and anti-PepB antibodies using line immunoassay. Applying previously proposed cut-offs for AhFibA, we obtained a sensitivity of 60.9% and a specificity of 98.7% in population 1. Receiver operating characteristic curve analysis could not detect a significant difference in diagnostic performance between the AhFibA ELISA and anti-CCP2 assay. Performing a hierarchical nearest neighborhood cluster analysis of the five different ACPA assays in population 2, we identified two clusters: a cluster of anti-pepA, anti-pepB and anti-CCP1, and a cluster of AhFibA and anti-CCP2. In conclusion, we found that AhFibA and anti-CCP2 antibodies had similar diagnostic performance. However, disagreement between ACPA tests may occur.

AbbreviationsACPA= anti-citrullinated protein-peptide antibody

ACR= American College of Rheumatology

AhFibA= anti-human fibrinogen autoantibodies

CCP= cyclic citrullinated peptide

ELISA= enzyme-linked immunosorbent assay

HRP= horseradish peroxidase

OD= optical density

PBS= phosphate-buffered saline

RA= rheumatoid arthritis

RF= rheumatoid factor

ROC= receiver operating curve.

Electronic supplementary materialThe online version of this article doi:10.1186-ar2011 contains supplementary material, which is available to authorized users.

Bert Vander Cruyssen, Tineke Cantaert contributed equally to this work.

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Autor: Bert Vander Cruyssen - Tineke Cantaert - Leonor Nogueira - Cyril Clavel - Leen De Rycke - Amélie Dendoven - Mireille Sebag

Fuente: https://link.springer.com/

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