The global establishment of a highly-fluoroquinolone resistant Salmonella enterica serotype Kentucky ST198 strainReport as inadecuate

The global establishment of a highly-fluoroquinolone resistant Salmonella enterica serotype Kentucky ST198 strain - Download this document for free, or read online. Document in PDF available to download.

* Corresponding author 1 BPE - Bactéries pathogènes entériques 2 FACULTY OF PHARMACY 3 Laboratoire de sécurité des aliments de Maisons-Alfort 4 Faculty of Veterinary Medicine 5 Unit AntiMicrobial Resistance and Resistance Determinants 6 Department of Microbiology 7 National Reference Centre for Salmonella and other Bacterial Enteric Pathogens 8 Centre for Infectious Diseases and Microbiology 9 Institut Pasteur du Maroc 10 University of Ibadan 11 Bio Chêne Vert 12 DTU - Technical University of Denmark Lyngby 13 Veterinary Pathobiology 14 IRBI - Institut de recherche sur la biologie de l-insecte 15 ISP - Infectiologie et Santé Publique

Abstract : While the spread of Salmonella enterica serotype Kentucky resistant to ciprofloxacin across Africa and the Middle-East has been described recently, the presence of this strain in humans, food, various animal species livestock, pets, and wildlife and in environment is suspected in other countries of different continents. Here, we report results of an in-depth molecular epidemiological study on a global human and non-human collection of S. Kentucky n = 70. We performed XbaI-pulsed field gel electrophoresis and multilocus sequence typing, assessed mutations in the quinolone resistance-determining regions, detected β-lactam resistance mechanisms, and screened the presence of the Salmonella genomic island 1 SGI1. In this study, we highlight the rapid and extensive worldwide dissemination of the ciprofloxacin-resistant S. Kentucky ST198-X1-SGI1 strain since the mid-2000s in an increasingly large number of contaminated sources, including the environment. This strain has accumulated an increasing number of chromosomal and plasmid resistance determinants and has been identified in the Indian subcontinent, Southeast Asia and Europe since 2010. The second substitution at position 87 in GyrA replacing the amino acid Asp appeared helpful for epidemiological studies to track the origin of contamination. This global study provides evidence leading to the conclusion that high-level resistance to ciprofloxacin in S. Kentucky is a simple microbiological trait that facilitates the identification of the epidemic clone of interest, ST198-X1-SGI1. Taking this into account is essential in order to detect and monitor it easily and to take rapid measures in livestock to ensure control of this infection.

Keywords : SGI1 poultry QRDR MDR Salmonella dissemination S Kentucky ST198

Author: Simon Le Hello - Amany Bekhit - Sophie A Granier - Himel Barua - Janine Beutlich - Magdalena Zaj - Sebastian Münch - Vitali Sint



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