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BMC Musculoskeletal Disorders

, 12:186

First Online: 15 August 2011Received: 08 March 2011Accepted: 15 August 2011


BackgroundPeriprosthetic osteolysis is a major cause of aseptic loosening in joint arthroplasty. This study investigates the impact of CT calcitonin deficiency and CT substitution under in-vivo circumstances on particle-induced osteolysis in Calca - mice.

MethodsWe used the murine calvarial osteolysis model based on ultra-high molecular weight polyethylene UHMWPE particles in 10 C57BL-6J wild-type WT mice and twenty Calca - mice. The mice were divided into six groups: WT without UHMWPE particles Group 1, WT with UHMWPE particles Group 2, Calca - mice without UHMWPE particles Group 3, Calca - mice with UHMWPE particles Group 4, Calca - mice without UHMWPE particles and calcitonin substitution Group 5, and Calca - mice with UHMWPE particle implantation and calcitonin substitution Group 6. Analytes were extracted from serum and urine. Bone resorption was measured by bone histomorphometry. The number of osteoclasts was determined by counting the tartrate-resistant acid phosphatase TRACP + cells.

ResultsBone resorption was significantly increased in Calca - mice compared with their corresponding WT. The eroded surface in Calca - mice with particle implantation was reduced by 20.6% after CT substitution. Osteoclast numbers were significantly increased in Calca - mice after particle implantation. Serum OPG osteoprotegerin increased significantly after CT substitution.

ConclusionsAs anticipated, Calca - mice show extensive osteolysis compared with wild-type mice, and CT substitution reduces particle-induced osteolysis.

List of AbbreviationsBVbone volume

CGRPcalcitonin gene-related peptide



LALLimulus Amebocyte Lysate

μ-CTmicro-computed tomography



ROIRegion of Interest

TRACPtartrate-resistant acid phosphatase

TRANCETNF-related activin-induced cytokine

UHMWPEultra-high molecular weight polyethylene


CTRcalcitonin receptor

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2474-12-186 contains supplementary material, which is available to authorized users.

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