Monitoring Proteins Using In Vivo Near-Infrared Time-Domain Optical Imaging after 2-O-Hexyldiglycerol-Mediated Transfer to the BrainReport as inadecuate




Monitoring Proteins Using In Vivo Near-Infrared Time-Domain Optical Imaging after 2-O-Hexyldiglycerol-Mediated Transfer to the Brain - Download this document for free, or read online. Document in PDF available to download.

Molecular Imaging and Biology

, Volume 13, Issue 2, pp 275–283

First Online: 03 June 2010

Abstract

PurposeThe aim of the present study was to gain insight into the penetration, biodistribution, and fate of globulins in the brain after 2-O-hexyldiglycerol-induced blood–brain barrier opening.

ProceduresThe spatial distribution of fluorescence probes was investigated after blood–brain barrier opening with intracarotid 2-O-hexyldiglycerol injection. Fluorescence intensity was visualized by microscopy mice and rats and by in vivo time-domain optical imaging.

ResultsThere was an increased 2-O-hexyldiglycerol-mediated transfer of fluorescence-labeled globulins into the ipsilateral hemisphere. Sequential in vivo measurements revealed that the increase in protein concentration lasted at least 96 h after administration. Ex vivo detection of tissue fluorescence confirmed the results obtained in vivo.

ConclusionGlobulins enter the healthy brain in conjunction with 2-O-hexyldiglycerol. Sequential in vivo near-infrared fluorescence measurements enable the visualization of the spatial distribution of antibodies in the brain of living small animals.

Key wordsBBB opening 2-O-Hexyldiglycerol Anti TGF-β antibody 1D11 Alexa Fluor 680 Cy5.5 Time-domain optical imaging The article shows the possibility to visualize antibodies in the brain of mice by near-infrared imaging after opening of the blood–brain barrier with 2-O-hexyldiglycerol.

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Author: Petra Hülper - Christian Dullin - Wilfried Kugler - Max Lakomek - Bernhard Erdlenbruch

Source: https://link.springer.com/







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