Human biodistribution and radiation dosimetry of novel PET probes targeting the deoxyribonucleoside salvage pathwayReport as inadecuate




Human biodistribution and radiation dosimetry of novel PET probes targeting the deoxyribonucleoside salvage pathway - Download this document for free, or read online. Document in PDF available to download.

European Journal of Nuclear Medicine and Molecular Imaging

, Volume 38, Issue 4, pp 711–721

First Online: 03 December 2010Received: 15 May 2010Accepted: 01 November 2010

Abstract

PurposeDeoxycytidine kinase dCK is a rate-limiting enzyme in deoxyribonucleoside salvage, a metabolic pathway involved in the production and maintenance of a balanced pool of deoxyribonucleoside triphosphates dNTPs for DNA synthesis. dCK phosphorylates and therefore activates nucleoside analogs such as cytarabine, gemcitabine, decitabine, cladribine, and clofarabine that are used routinely in cancer therapy. Imaging probes that target dCK might allow stratifying patients into likely responders and nonresponders with dCK-dependent prodrugs. Here we present the biodistribution and radiation dosimetry of three fluorinated dCK substrates, F-FAC, L-F-FAC, and L-F-FMAC, developed for positron emission tomography PET imaging of dCK activity in vivo.

MethodsPET studies were performed in nine healthy human volunteers, three for each probe. After a transmission scan, the radiopharmaceutical was injected intravenously and three sequential emission scans acquired from the base of the skull to mid-thigh. Regions of interest encompassing visible organs were drawn on the first PET scan and copied to the subsequent scans. Activity in target organs was determined and absorbed dose estimated with OLINDA-EXM. The standardized uptake value was calculated for various organs at different times.

ResultsRenal excretion was common to all three probes. Bone marrow had higher uptake for L-F-FAC and L-F-FMAC than F-FAC. Prominent liver uptake was seen in L-F-FMAC and L-F-FAC, whereas splenic activity was highest for F-FAC. Muscle uptake was also highest for F-FAC. The critical organ was the bladder wall for all three probes. The effective dose was 0.00524, 0.00755, and 0.00910 mSv-MBq for F-FAC, L-F-FAC, and L-F-FMAC, respectively.

ConclusionThe biodistribution of F-FAC, L-F-FAC, and L-F-FMAC in humans reveals similarities and differences. Differences may be explained by different probe affinities for nucleoside transporters, dCK, and catabolic enzymes such as cytidine deaminase CDA. Dosimetry demonstrates that all three probes can be used safely to image the deoxyribonucleoside salvage pathway in humans.

KeywordsDeoxyribonucleoside salvage pathway Deoxycytidine kinase PET imaging FAC PET Dosimetry Electronic supplementary materialThe online version of this article doi:10.1007-s00259-010-1666-z contains supplementary material, which is available to authorized users.

Download fulltext PDF



Author: Johannes Schwarzenberg - Caius G. Radu - Matthias Benz - Barbara Fueger - Andrew Q. Tran - Michael E. Phelps - Owen N. 

Source: https://link.springer.com/







Related documents