Changes in the expression of aromatase, estrogen receptor α and β in mandibular condylar cartilage of rats induced by disordered occlusionReport as inadecuate




Changes in the expression of aromatase, estrogen receptor α and β in mandibular condylar cartilage of rats induced by disordered occlusion - Download this document for free, or read online. Document in PDF available to download.

BMC Musculoskeletal Disorders

, 13:190

First Online: 30 September 2012Received: 28 November 2011Accepted: 26 September 2012

Abstract

BackgroundEstrogens play an important role in modulating the morphology and function of temporomandibular joints TMJs, which is suggested to act via estrogen receptors ERs. The present study was to investigate the expression of aggrecan, collagen type II Col II, Col X, aromatase, ERα and ERβ in degenerative changes of mandibular condylar cartilage.

MethodsForty male and 40 female 8-week-old rats were enrolled in this study. In experimental groups, the disordered occlusion was created by moving the first molars mesially and the third ones distally. Immunohistochemistry and real-time PCR were performed at the end of the second or fourth week.

ResultsDegenerative changes, characterized by interrupted continuity of hypertrophic layer, pyknotic and eosinophilic lesion with few nuclei, areas filled with eosinophilic nuclei, were observed in more joints from female experimental groups than male ones. However, thickening changes in hypertrophic layer were only found in male experimental groups. The gene expression of Col II, Col X and aggrecan increased in 4-wk male experimental subgroup both P < 0.01, but decreased in 2-wk and 4-wk female subgroups P < 0.05. The gene expression of ERα decreased in 2-wk male and female experimental subgroups both P < 0.01, however, that of ERβ increased except the 2-wk female experimental subgroup all P < 0.01. The expression of aromatase decreased in both male and female experimental subgroups all P<0.01.

ConclusionsMandibular condylar cartilage responses differently to the disordered occlusion in male and female rats. The levels of locally synthesized estrogen, ERα and ERβ may have limited attribution, if any, to the sex-specific cartilage response.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2474-13-190 contains supplementary material, which is available to authorized users.

Download fulltext PDF



Author: Shibin Yu - Xianghui Xing - Kai Jiao - Lei Sun - Lei Liu - Meiqing Wang

Source: https://link.springer.com/







Related documents