Renal denervation in an animal model of diabetes and hypertension: Impact on the autonomic nervous system and nephropathyReport as inadecuate

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Cardiovascular Diabetology

, 10:33

First Online: 17 April 2011Received: 06 February 2011Accepted: 17 April 2011


BackgroundThe effects of renal denervation on cardiovascular reflexes and markers of nephropathy in diabetic-hypertensive rats have not yet been explored.

MethodsAim: To evaluate the effects of renal denervation on nephropathy development mechanisms blood pressure, cardiovascular autonomic changes, renal GLUT2 in diabetic-hypertensive rats. Forty-one male spontaneously hypertensive rats SHR ~250 g were injected with STZ or not; 30 days later, surgical renal denervation RD or sham procedure was performed; 15 days later, glycemia and albuminuria ELISA were evaluated. Catheters were implanted into the femoral artery to evaluate arterial pressure AP and heart rate variability spectral analysis one day later in conscious animals. Animals were killed, kidneys removed, and cortical renal GLUT2 quantified Western blotting.

ResultsHigher glycemia p < 0.05 and lower mean AP were observed in diabetics vs. nondiabetics p < 0.05. Heart rate was higher in renal-denervated hypertensive and lower in diabetic-hypertensive rats 384.8 ± 37, 431.3 ± 36, 316.2 ± 5, 363.8 ± 12 bpm in SHR, RD-SHR, STZ-SHR and RD-STZ-SHR, respectively. Heart rate variability was higher in renal-denervated diabetic-hypertensive rats 55.75 ± 25.21, 73.40 ± 53.30, 148.4 ± 93 in RD-SHR, STZ-SHR- and RD-STZ-SHR, respectively, p < 0.05, as well as the LF component of AP variability 1.62 ± 0.9, 2.12 ± 0.9, 7.38 ± 6.5 in RD-SHR, STZ-SHR and RD-STZ-SHR, respectively, p < 0.05. GLUT2 renal content was higher in all groups vs. SHR.

ConclusionsRenal denervation in diabetic-hypertensive rats improved previously reduced heart rate variability. The GLUT2 equally overexpressed by diabetes and renal denervation may represent a maximal derangement effect of each condition.

Electronic supplementary materialThe online version of this article doi:10.1186-1475-2840-10-33 contains supplementary material, which is available to authorized users.

Lucinara D Dias, Karina R Casali, Natalia M Leguisamo, Felipe Azambuja, Martina S Souza, Maristela Okamoto, Ubiratan F Machado, Maria Cláudia Irigoyen and Beatriz D Schaan contributed equally to this work.

An erratum to this article is available at

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Author: Lucinara D Dias - Karina R Casali - Natalia M Leguisamo - Felipe Azambuja - Martina S Souza - Maristela Okamoto - Ubirat


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