Changes in cardiac heparan sulfate proteoglycan expression and streptozotocin-induced diastolic dysfunction in ratsReport as inadecuate




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Cardiovascular Diabetology

, 10:35

First Online: 25 April 2011Received: 22 February 2011Accepted: 25 April 2011

Abstract

BackgroundChanges in the proteoglycans glypican and syndecan-4 have been reported in several pathological conditions, but little is known about their expression in the heart during diabetes. The aim of this study was to investigate in vivo heart function changes and alterations in mRNA expression and protein levels of glypican-1 and syndecan-4 in cardiac and skeletal muscles during streptozotocin STZ-induced diabetes.

MethodsDiabetes was induced in male Wistar rats by STZ administration. The rats were assigned to one of the following groups: control sham injection, after 24 hours, 10 days, or 30 days of STZ administration. Echocardiography was performed in the control and STZ 10-day groups. Western and Northern blots were used to quantify protein and mRNA levels in all groups. Immunohistochemistry was performed in the control and 30-day groups to correlate the observed mRNA changes to the protein expression.

ResultsIn vivo cardiac functional analysis performed using echocardiography in the 10-day group showed diastolic dysfunction with alterations in the peak velocity of early E diastolic filling and isovolumic relaxation time IVRT indices. These functional alterations observed in the STZ 10-day group correlated with the concomitant increase in syndecan-4 and glypican-1 protein expression. Cardiac glypican-1 mRNA and skeletal syndecan-4 mRNA and protein levels increased in the STZ 30-day group. On the other hand, the amount of glypican in skeletal muscle was lower than that in the control group. The same results were obtained from immunohistochemistry analysis.

ConclusionOur data suggest that membrane proteoglycans participate in the sequence of events triggered by diabetes and inflicted on cardiac and skeletal muscles.

KeywordsGlypican Syndecan-4 Diabetes Cardiac Muscle List of abbreviationsb-FGFbasic fibroblast growth factor

DTdeceleration time of the E wave

HSPGheparan sulfate proteoglycan

IVRTisovolumic relaxation time

IVSTinterventricular septal thickness

LVEDDend-diastolic dimension

LVESDleft ventricular end-systolic

PWTposterior wall thickness

STZstreptozotocin

ZDFZucker diabetic fatty rats.

Electronic supplementary materialThe online version of this article doi:10.1186-1475-2840-10-35 contains supplementary material, which is available to authorized users.

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Source: https://link.springer.com/article/10.1186/1475-2840-10-35







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