Effect of autologous platelet-rich plasma-releasate on intervertebral disc degeneration in the rabbit anular puncture model: a preclinical studyReport as inadecuate

Effect of autologous platelet-rich plasma-releasate on intervertebral disc degeneration in the rabbit anular puncture model: a preclinical study - Download this document for free, or read online. Document in PDF available to download.

Arthritis Research and Therapy

, 14:R241

First Online: 05 November 2012Received: 26 July 2012Revised: 13 October 2012Accepted: 02 November 2012


IntroductionPlatelet-rich plasma PRP is a fraction of plasma in which several growth factors are concentrated at high levels. The active soluble releasate isolated following platelet activation of PRP PRP-releasate has been demonstrated to stimulate the metabolism of IVD cells in vitro. The in vivo effect of PRP-releasate on degenerated IVD remains unknown. The purpose of this study was to determine the reparative effects of autologous PRP-releasate on degenerated intervertebral discs IVDs.

MethodsTo induce disc degeneration, New Zealand white rabbits n = 12 received anular puncture in two noncontiguous discs. Autologous PRP and PPP platelet-poor plasma were isolated from fresh blood using two centrifugation techniques. Four weeks after the initial puncture, releasate isolated from clotted PPP or PRP PPP- or PRP-releasate, or phosphate-buffered saline PBS; control was injected into the punctured discs. Disc height, magnetic resonance imaging MRI T2-mapping and histology were assessed.

ResultsAnular puncture produced a consistent disc narrowing within four weeks. PRP-releasate induced a statistically significant restoration of disc height PRP vs. PPP and PBS, P<0.05. In T2-quantification, the mean T2-values of the nucleus pulposus NP and anulus fibrosus AF of the discs were not significantly different among the three treatment groups. Histologically, the number of chondrocyte-like cells was significantly higher in the discs injected with PRP-releasate compared to that with PBS.

ConclusionsThe administration of active PRP-releasate induced a reparative effect on rabbit degenerated IVDs. The results of this study suggest that the use of autologous PRP-releasate is safe and can lead to a clinical application for IVD degeneration.

AbbreviationsAFanulus fibrosus

ANOVAanalysis of variance

BMPbone morphogenetic protein

DHIdisc height index

EGFepidermal growth factor

GDF-5growth differentiation factor-5

IGF-1insulin-like growth factor-1


IVDintervertebral disc

MRImagnetic resonance imaging

NPnucleus pulposus

OP-1osteogenic protein-1

PBSphosphate-buffered saline

PDGFplatelet-derived growth factor

PPPplatelet-poor plasma

PRPplatelet-rich plasma

PRP-GHMplatelet-rich plasma-impregnated biodegradable gelatin hydrogel microsphere

ROIregion of interest


TGF-βtransforming growth factor-β.

Electronic supplementary materialThe online version of this article doi:10.1186-ar4084 contains supplementary material, which is available to authorized users.

Shuji Obata, Koji Akeda contributed equally to this work.

Download fulltext PDF

Author: Shuji Obata - Koji Akeda - Takao Imanishi - Koichi Masuda - Won Bae - Ryo Morimoto - Yumiko Asanuma - Yuichi Kasai - Atsum

Source: https://link.springer.com/

Related documents