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EJNMMI Research

, 1:3

First Online: 07 June 2011Received: 15 February 2011Accepted: 07 June 2011

Abstract

BackgroundReceptor for advanced glycation endproducts RAGE expression contributes to the impaired angiogenic response to limb ischemia in diabetes. The aim of this study was to detect the effect of increased expression of RAGE on the angiogenic response to limb ischemia in diabetes by targeting αvβ3 integrin with Tc-labeled Arg-Gly-Asp RGD.

MethodsMale wild-type WT C57BL-6 mice were either made diabetic or left as control for 2 months when they underwent femoral artery ligation. Four groups were studied at days 3 to 7 after ligation: WT without diabetes NDM n = 14, WT with diabetes DM n = 14, RAGE NDM n = 16, and RAGE DM n = 14. Mice were injected with Tc-HYNIC-RGD and imaged. Count ratios for ischemic-non-ischemic limbs were measured. Muscle was stained for RAGE, αvβ3, and lectins.

ResultsThere was no difference in count ratio between RAGE and WT NDM groups. Mean count ratio was lower for WT DM 1.38 ± 0.26 vs. WT NDM 1.91 ± 0.34 P<0.001. Mean count ratio was lower for the RAGE DM group than for RAGE NDM group 1.75 ± 0.22 vs. 2.02 ± 0.29 P<0.001 and higher than for the WT DM group P<0.001. Immunohistopathology supported the scan findings.

ConclusionsIn vivo imaging of αvβ3 integrin can detect the effect of RAGE on the angiogenic response to limb ischemia in diabetes.

AbbreviationsAGEsadvanced glycation endproducts

DMdiabetes mellitus

FAfemoral artery

MMPsmatrix metalloproteinases

NDMnon-diabetes mellitus

%ID-gpercent injected dose per gram

ROIregion of interest

RAGEreceptor for advanced glycation endproducts

VEGFvascular endothelial growth factor

WTwild-type.

Electronic supplementary materialThe online version of this article doi:10.1186-2191-219X-1-3 contains supplementary material, which is available to authorized users.

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Author: Yared Tekabe - Xiaoping Shen - Joane Luma - Drew Weisenberger - Shi Fang Yan - Roland Haubner - Ann Marie Schmidt - Lynne

Source: https://link.springer.com/







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