Effect of ischemic preconditioning in skeletal muscle measured by functional magnetic resonance imaging and spectroscopy: a randomized crossover trialReport as inadecuate




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Journal of Cardiovascular Magnetic Resonance

, 13:32

First Online: 30 June 2011Received: 15 February 2011Accepted: 30 June 2011

Abstract

BackgroundNuclear magnetic resonance NMR imaging and spectroscopy have been applied to assess skeletal muscle oxidative metabolism. Therefore, in-vivo NMR may enable the characterization of ischemia-reperfusion injury. The goal of this study was to evaluate whether NMR could detect the effects of ischemic preconditioning IPC in healthy subjects.

MethodsTwenty-three participants were included in two randomized crossover protocols in which the effects of IPC were measured by NMR and muscle force assessments. Leg ischemia was administered for 20 minutes with or without a subsequent impaired reperfusion for 5 minutes stenosis model. IPC was administered 4 or 48 hours prior to ischemia. Changes in phosphate NMR spectroscopy and blood oxygen level-dependent BOLD signals were recorded. 3-Tesla NMR data were compared to those obtained for isometric muscular strength.

ResultsThe phosphocreatine PCr signal decreased robustly during ischemia and recovered rapidly during reperfusion. In contrast to PCr, the recovery of muscular strength was slow. During post-ischemic stenosis, PCr increased only slightly. The BOLD signal intensity decreased during ischemia, ischemic exercise and post-ischemic stenosis but increased during hyperemic reperfusion. IPC 4 hours prior to ischemia significantly increased the maximal PCr reperfusion signal and mitigated the peak BOLD signal during reperfusion.

ConclusionsIschemic preconditioning positively influenced muscle metabolism during reperfusion; this resulted in an increase in PCr production and higher oxygen consumption, thereby mitigating the peak BOLD signal. In addition, an impairment of energy replenishment during the low-flow reperfusion was detected in this model. Thus, functional NMR is capable of characterizing changes in reperfusion and in therapeutic interventions in vivo.

Trial RegistrationClinicalTrials.gov: NCT00883467

Electronic supplementary materialThe online version of this article doi:10.1186-1532-429X-13-32 contains supplementary material, which is available to authorized users.

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Author: Martin Andreas - Albrecht I Schmid - Mohammad Keilani - Daniel Doberer - Johann Bartko - Richard Crevenna - Ewald Moser -

Source: https://link.springer.com/article/10.1186/1532-429X-13-32







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