Phenotypic characterization of skeletal abnormalities of Osteopotentia mutant mice by micro-CT: a descriptive approach with emphasis on reconstruction techniquesReport as inadecuate




Phenotypic characterization of skeletal abnormalities of Osteopotentia mutant mice by micro-CT: a descriptive approach with emphasis on reconstruction techniques - Download this document for free, or read online. Document in PDF available to download.

Skeletal Radiology

, Volume 40, Issue 8, pp 1073–1078

First Online: 05 January 2011Received: 17 September 2010Revised: 02 December 2010Accepted: 07 December 2010

Abstract

PurposeThe novel protein osteopotentia Opt has recently been described as an essential regulator of postnatal osteoblast maturation and might possibly be responsible for some of the rarer types of osteogenesis imperfecta. Our aim was the evaluation of micro CT for the qualitative morphological assessment of skeletal abnormalities of Osteopotentia-mutant mice in comparison to radiography and histology.

Materials and methodsFour homozygous mice with insertional mutations in the Opt gene and three wild-type controls were examined ex vivo using radiography and micro-CT. Two of the homozygous animals were evaluated histologically trichrome reagent. For the micro-CT evaluation three-dimensional 3D surface reconstructions and two-dimensional 2D multiplanar reformations MPRs were applied.

ResultsThe Opt-homozygous mice exhibited severe growth. The radiographic examinations showed osteopenia and fractures with hypertrophic callus formation and pseudarthroses of the forelimbs and ribs. Micro-CT confirmed these findings and was able to demonstrate additional fractures especially at smaller bones such as the metacarpals and phalanges. Additional characterization and superior delineation of cortices and fracture fragments was achieved by 2D MPRs. Histological correlation verified several of these imaging findings.

ConclusionMicro-CT is able to screen Opt-mutant mice for osseous pathologies and furthermore characterize these anomalies. The modality seems superior to conventional radiography, but is not able to demonstrate cellular pathology. However, histology is destructive and more time- and material-consuming than micro-CT. Additional information may be gathered by 2D MPRs.

KeywordsAnimal model Micro CT Bone Radiography Fracture Osteogenesis imperfecta  Download fulltext PDF



Author: Frank W. Roemer - Andreas Mohr - Ali Guermazi - Yebin Jiang - Philipp Schlechtweg - Harry K. Genant - Michael L. Sohaske

Source: https://link.springer.com/







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