Uptake of 68gallium in atherosclerotic plaques in LDLR-ApoB100-100 miceReport as inadecuate




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EJNMMI Research

, 1:14

First Online: 17 August 2011Received: 06 July 2011Accepted: 17 August 2011

Abstract

BackgroundAtherosclerosis is a chronic inflammatory disease of artery wall characterized by infiltration of monocytes into subendothelial space and their differentiation into macrophages. Since rupture-prone plaques commonly contain high amounts of activated macrophages, imaging of the macrophage content may provide a useful tool for the evaluation of plaque vulnerability. The purpose of this study was to explore the uptake of gallium Ga in atherosclerotic plaques in mice.

MethodsUptake of ionic Ga was investigated in atherosclerotic LDLRApoB and C57BL-6N control mice at 3 h after injection. The ex vivo biodistribution of the Ga was assessed and autoradiography of aortic cryosections was defined. In vivo imaging of Ga was performed using a small animal positron emission tomography PET-CT scanner.

ResultsOur results revealed that the uptake of Ga-radioactivity was higher in atherosclerotic plaques than in healthy vessel wall ratio 1.8 ± 0.2, p = 0.0002 and adventitia ratio 1.3 ± 0.2, p = 0.0011. The autoradiography signal co-localized with macrophages prominently as demonstrated by Mac-3 staining. In both mice strains, the highest level of radioactivity was found in the blood.

ConclusionsWe observed a moderate but significantly elevated Ga-radioactivity uptake in the aortic plaques of atherosclerotic mice, especially at the sites rich in macrophages. While the uptake of Ga was promising in this animal model, the slow blood clearance may limit the usability of Ga as a PET tracer for clinical imaging of atherosclerotic plaques.

Keywordsatherosclerosis plaque autoradiography gallium-68 PET Electronic supplementary materialThe online version of this article doi:10.1186-2191-219X-1-14 contains supplementary material, which is available to authorized users.

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Author: Johanna MU Silvola - Iina Laitinen - Henri J Sipilä - V Jukka O Laine - Pia Leppänen - Seppo Ylä-Herttuala - Juhani 

Source: https://link.springer.com/







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