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Rheumatology International

, Volume 33, Issue 1, pp 37–43

First Online: 04 January 2012Received: 22 June 2011Accepted: 08 December 2011

Abstract

To find whether the plasma fibronectin FN molecular status can be useful to differentiate between rheumatoid arthritis RA and systemic lupus erythematosus SLE. The expression of plasma FN domains was determined by ELISA using monoclonal domain-specific antibodies. FN molecular forms were revealed by immunoblotting and analyzed by densitometry. The following findings were found: 1 Mean values of FN concentration were lower in SLE and RA patients than in normal plasmas. The cut off points at 31 mg-l in SLE and at 45 mg-l in RA showed a sensitivity and specificity of 54, 55 and 75%, respectively. 2 Mean values of concentrations of FN and FN were lower in SLE than those in normal and RA plasmas. Quantified data showed the cut off points of FN and FN at 200 mg-l 58% of sensitivity, 56% of specificity and 350 mg-l 58% of sensitivity, 58% of specificity in SLE, as well as at 295 mg-l 52% of sensitivity, 51% of specificity and 460 mg-l in RA 70% of sensitivity, 73% of specificity. 3 The plasma FN immunopatterns, characterized by the presence of high-molecular 260–310 kDa and-or low-molecular 158–209 kDa FN bands, were specific only for SLE samples. The analysis of plasma FN status revealed by its Fibrin-Heparin-, CBD- and Ct-domain reactivity with monoclonal antibody and immunoblotting can be helpful to differentiate the SLE in respect to RA and normal plasmas.

KeywordsFibronectin Fibronectin fragments Fibronectin domains Rheumatoid arthritis Systemic lupus erythematosus  Download fulltext PDF



Autor: Magdalena Przybysz - Krzysztof Borysewicz - Iwona Kątnik-Prastowska

Fuente: https://link.springer.com/







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