Specific tumor-stroma interactions of EBV-positive Burkitts lymphoma cells in the chick chorioallantoic membraneReportar como inadecuado




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Vascular Cell

, 4:3

First Online: 09 March 2012Received: 26 January 2012Accepted: 09 March 2012

Abstract

BackgroundBurkitt-s lymphoma BL is an aggressive Non-Hodgkin lymphoma. Epstein-Barr Virus EBV is able to transform B cells and is a causative infectious agent in BL. The precise role of EBV in lymphoma progression is still unclear. Most investigations have concentrated on cell autonomous functions of EBV in B cells. Functions of the local environment in BL progression have rarely been studied, mainly due to the lack of appropriate in vivo models. Therefore, we inoculated different human BL cell-lines onto the chorioallantoic membrane CAM of embryonic day 10 ED10 chick embryos and re-incubated until ED14 and ED17.

ResultsAll cell-lines formed solid tumors. However, we observed strong differences in the behavior of EBV and EBV cell-lines. Tumor borders of EBV cells were very fuzzy and numerous cells migrated into the CAM. In EBV tumors, the borders were much better defined. In contrast to EBV cells, the EBV cells induced massive immigration of chick leukocytes at the tumor borders and the development of granulation tissue with large numbers of blood vessels and lymphatics, although the expression of pro- and anti-angiogenic forms of Vascular Endothelial Growth Factors-receptors was the same in all BL cell-lines tested. The EBV cell-lines massively disseminated via the lymphatics and completely occluded them.

ConclusionsOur data suggest that the EBV cells attract pro-angiogenic leukocytes, which then induce secondary tumor-stroma interactions contributing to the progression of BL. We show that the CAM is a highly suitable in vivo model to study the differential behavior of BL cell-lines.

KeywordsBurkitt-s lymphoma EBV BL2 BL2B95 BL74 Lymphatics Dissemination VEGF-A VEGF-C esVEGFR-2 Electronic supplementary materialThe online version of this article doi:10.1186-2045-824X-4-3 contains supplementary material, which is available to authorized users.

Jürgen Becker, Ana Covelo-Fernandez contributed equally to this work.

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Autor: Jürgen Becker - Ana Covelo-Fernandez - Frederike von Bonin - Dieter Kube - Jörg Wilting

Fuente: https://link.springer.com/article/10.1186/2045-824X-4-3







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