Leptin Administration Downregulates the Increased Expression Levels of Genes Related to Oxidative Stress and Inflammation in the Skeletal Muscle of ob-ob MiceReportar como inadecuado




Leptin Administration Downregulates the Increased Expression Levels of Genes Related to Oxidative Stress and Inflammation in the Skeletal Muscle of ob-ob Mice - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Mediators of InflammationVolume 2010 2010, Article ID 784343, 15 pages

Research Article

Metabolic Research Laboratory, Clínica Universidad de Navarra 3, 31008 Pamplona, Spain

CIBER Fisiopatología de la Obesidad y Nutrición CIBEROBN, Instituto de Salud Carlos III, Spain

Department of Endocrinology, Clínica Universidad de Navarra, Pío XII 36, 31008 Pamplona, Spain

Received 21 January 2010; Revised 31 March 2010; Accepted 24 April 2010

Academic Editor: Giamila Fantuzzi

Copyright © 2010 Neira Sáinz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Obese leptin-deficient ob-ob mice exhibit alow-grade chronic inflammation together with a low muscle mass. Our aim was to analyze the changes in muscle expression levels ofgenes related to oxidative stress and inflammatory responses inleptin deficiency and to identify the effect of invivo leptin administration. Ob-ob micewere divided in three groups as follows: controlob-ob, leptin-treated ob-ob1 mg-kg-d and leptin pair-fed ob-obmice. Gastrocnemius weight was lower in controlob-ob than in wild type mice exhibiting an increase after leptin treatmentcompared to control and pair-fed ob-ob animals. Thiobarbituric acidreactive substances, markers of oxidative stress, were higher inserum and gastrocnemius of control ob-ob than in wild typemice and were significantly decreased by leptin treatment. Leptin deficiency altered theexpression of 1,546 genes, while leptin treatment modified theregulation of 1,127 genes with 86 of them being involved inoxidative stress, immune defense and inflammatory response. Leptinadministration decreased the high expression of Crybb1,Hspb3, Hspb7, Mt4, Cat, Rbm9, Serpinc1 andSerpinb1a observed in controlob-ob mice, indicating that it improvesinflammation and muscle loss.





Autor: Neira Sáinz, Amaia Rodríguez, Victoria Catalán, Sara Becerril, Beatriz Ramírez, Javier Gómez-Ambrosi, and Gema Frühbec

Fuente: https://www.hindawi.com/



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