Anti-cyclic citrullinated peptide antibodies and paraoxonase-1 polymorphism in rheumatoid arthritisReportar como inadecuado

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BMC Musculoskeletal Disorders

, 15:379

Pathophysiology of musculoskeletal disorders


BackgroundRheumatoid arthritis RA is the most common chronic inflammatory joint disease, with a worldwide prevalence of 0.5% to 1%. Anti-cyclic citrullinated peptide antibody anti-CCP-2 Ab is a marker of choice for diagnosing early and late RA. Anti-oxidant enzymes activity decreases in RA patients. Till now, the relationship between the rheumatoid factor RF and anti-CCP-2 Ab, anti-oxidant activity and polymorphism of paraoxenase-1 PON-1 192 Q-R in patients with RA has not been investigated. In this study, we aimed to determine the serum level of RF and anti-CCP-2 Ab, PON-1 activity and 192 Q-R polymorphism and arylesterase ARE activity in patients with RA. Also, we studied RA markers in different genotypes of PON-1 of RA patients.

MethodsA total of 120 RA patients and 90 healthy persons were subjected to full clinical examinations and routine laboratory tests. PON-1 and ARE activities were determined using an enzymatic spectrophotometric method. PON-1 192 gene polymorphism was determined using polymerase chain reaction based restriction fragment analysis. RF was measured by immunoturbidimetry method and anti-CCP-2 Ab was assayed by enzyme-linked immunosorbent assay ELISA. Statistical analysis was performed using SPSS for windows 20.0.

ResultsThe sensitivity and specificity of anti-CCP-2 Ab for the diagnosis of RA were 76.2% and 100% respectively. PON-1 and ARE activities were statistically lower P <0.001 in the RA group compared to the control group. A negative correlation between RF and anti-CCP-2 Ab levels and PON-1 and ARE activities was found. No significant difference in the genotype distribution between RA patients and healthy persons was detected. RF and anti-CCP-2 Ab levels were higher in RA patients carried RR genotype than in those carried QQ genotype.

ConclusionHigh RF and anti-CCP-2 antibody serum levels were found to be associated with decreased PON-1 and ARE activities with no correlation between PON-1 polymorphism and serum levels of RF and anti-CCP-2 Ab in patients with RA. These results may indicate an implication between antioxidant enzymes activity and serum levels of RF and anti-CCP-2 Ab.

KeywordsRheumatoid arthritis Anti-CCP-2 antibodies Paraoxonase-1 polymorphism Rheumatoid factor Arylesterase  Download fulltext PDF

Autor: Hassan El-Banna - Asif Jiman-Fatani


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