Glyceraldehyde-derived pyridinium GLAP evokes oxidative stress and inflammatory and thrombogenic reactions in endothelial cells via the interaction with RAGEReport as inadecuate

Glyceraldehyde-derived pyridinium GLAP evokes oxidative stress and inflammatory and thrombogenic reactions in endothelial cells via the interaction with RAGE - Download this document for free, or read online. Document in PDF available to download.

Cardiovascular Diabetology

, 14:1

First Online: 08 January 2015Received: 20 October 2014Accepted: 04 December 2014


BackgroundWe have previously shown that serum levels of glyceraldehyde-derived advanced glycation end products Gly-AGEs are elevated under oxidative stress and-or diabetic conditions and associated with insulin resistance, endothelial dysfunction and vascular inflammation in humans. Further, Gly-AGEs not only evoke oxidative and inflammatory reactions in endothelial cells ECs through the interaction with a receptor for AGEs RAGE, but also mimic vasopermeability effects of AGE-rich serum purified from diabetic patients on hemodialysis. These observations suggest that Gly-AGE-RAGE system might be a therapeutic target for vascular complications in diabetes. However, since incubation of glyceraldehyde with proteins will generate a large number of structurally distinct AGEs, it remains unclear what type of AGE structures could mediate the deleterious effects of Gly-AGEs on ECs.

Aims and MethodsTherefore, in this study, we examined 1 whether glyceraldehyde-derived pyridinium GLAP, one of the Gly-AGEs generated by the incubation of lysine with glyceraldehyde, elicited reactive oxygen species ROS generation and inflammatory and thrombogenic gene expression in human umbilical vein ECs HUVECs via the interaction with RAGE and 2 if DNA aptamers raised against Gly-AGEs or GLAP AGE-aptamer or GLAP-aptamer inhibited the binding of GLAP to RAGE and subsequently suppressed the harmful effects of GLAP on HUVECs.

ResultsGLAP stimulated ROS generation in a bell-shaped manner; GLAP at 10 μg-ml increased ROS generation in HUVECs by 40%, which was blocked by the treatment with RAGE-antibody RAGE-Ab. Ten μg-ml GLAP significantly up-regulated mRNA levels of RAGE, monocyte chemoattractant protein-1, intercellular adhesion molecule-1, vascular cell adhesion molecule-1 and plasminogen activator inhibitor-1 in HUVECs, which were also suppressed by RAGE-Ab. AGE-aptamer or GLAP-aptamer significantly blocked these deleterious effects of GLAP on HUVECs. Moreover, quartz crystal microbalance analyses revealed that GLAP actually bound to RAGE and that AGE-aptamer or GLAP-aptamer inhibited the binding of GLAP to RAGE.

ConclusionsThe present study suggests that GLAP might be a main glyceraldehyde-related AGE structure in Gly-AGEs that bound to RAGE and subsequently elicited ROS generation and inflammatory and thrombogenic reactions in HUVECs. Blockade of the GLAP-RAGE interaction by AGE-aptamer or GLAP-aptamer might be a novel therapeutic strategy for preventing vascular injury in diabetes.

KeywordsAGEs RAGE GLAP Aptamer Oxidative stress AbbreviationsAGEsAdvanced glycation end products

RAGEReceptor for AGEs

Gly-AGEsGlyceraldehyde-derived advanced glycation end products

ECsEndothelial cells

SELEXSystematic evolution of ligands by exponential enrichment

AGE-aptamerDNA aptamer directed against Gly-AGEs

ROSReactive oxygen species

HUVECsHuman umbilical vein ECs

GLAPGlyceraldehyde-derived pyridinium

GLAP-aptamerDNA aptamer directed against GLAP

BSABovine serum albumin

HPLCHigh-performance liquid chromatography

RAGE-AbAntibody directed against human RAGE

PCRPolymerase chain reaction

PBSPhosphate-buffered saline

Control-aptamerControl DNA aptamer

DMSODimethyl sulfoxide

RT-PCRReverse transcription-PCR

MCP-1Monocyte chemoattractant protein-1

VCAM-1Vascular cell adhesion molecule-1

ICAM-1Intercellular adhesion molecule-1

PAI-1Plasminogen activator inhibitor-1

vRAGEextracellular AGE-binding v-domain of RAGE

QCMQuartz crystal microbalance

KDDissociation constant

NF-κBNuclear factor-κB

GAPDHGlyceraldehyde-3-phosphate dehydrogenase

PI3KPhosphatidylinositol 3-phosphate kinase

mTORmammalian target of rapamycin.

Download fulltext PDF

Author: Takanori Matsui - Eriko Oda - Yuichiro Higashimoto - Sho-ichi Yamagishi


Related documents