Inhibiting HMGB1 Reduces Cerebral Ischemia Reperfusion Injury in Diabetic MiceReportar como inadecuado

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, Volume 39, Issue 6, pp 1862–1870

First Online: 05 September 2016


High mobility group box1 HMGB1 promotes inflammatory injury, and accumulating evidence suggests that it plays a key role in brain ischemia reperfusion I-R, as well as the development of diabetes mellitus DM. The purpose of this study was to investigate whether HMGB1 plays a role in brain I-R in a DM mouse model. Diabetes mellitus was induced by a high-calorie diet and streptozotocin treatment, and cerebral ischemia was induced by middle cerebral artery occlusion. We examined HMGB1 levels following cerebral I-R injury in DM and non-DM mice and evaluated the influence of altered HMGB1 levels on the severity of cerebral injury. Serum HMGB1 levels and the inflammatory factors IL-1β, IL-6, and inflammation-related enzyme iNOS were significantly elevated in DM mice with brain I-R compared with non-DM mice with brain I-R. Blocking HMGB1 function by intraperitoneal injection of anti-HMGB1 neutralizing antibodies reversed the inflammatory response and the extent of brain damage, suggesting that HMGB1 plays an important role in cerebral ischemic stroke in diabetic mice.

KEY WORDSHMGB1 brain ischemia reperfusion diabetes mellitus AbbreviationsHMGB1High mobility group box1

I-RIschemia reperfusion

DMDiabetes mellitus

mAbMonoclonal antibody

MCAOMiddle cerebral artery occlusion

CCACommon carotid artery

ICAInternal carotid artery

ECAExternal carotid artery

ELISAEnzyme-linked immunosorbent assay

BBBBlood–brain barrier

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Autor: Chong Wang - Jie Jiang - Xiuping Zhang - Linjie Song - Kai Sun - Ruxiang Xu


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