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Journal of Cardiovascular Magnetic Resonance

, 18:19

First Online: 16 April 2016Received: 15 January 2016Accepted: 31 March 2016


BackgroundCardiovascular magnetic resonance offers both diagnostic and prognostic information in myocarditis. Using an established animal model of myocarditis, the aim of this study was to measure myocardial T1 before the onset, in the acute and in the chronic phases of the disease and to compare its course with histological and immunohistochemistry findings.

MethodsMale young Lewis rats were immunized with 0.25 mg porcine myocardial myosin into the rear footpads on day 0. Native and contrast-enhanced ECG-triggered cardiac MRI examinations were performed before immunization on day 0 and on days 14, 21 and 35. Left ventricular function, pre- and post- contrast T1 parameters and LGE images were assessed using Small animal look-locker inversion recovery SALLI. For each of the indicated time points a minimum of 4 rats were randomly sacrificed for pathological investigations including conventional histology HE and Sirius-Red staining and immunohistochemistry CD 68 investigations.

ResultsAll immunized rats developed myocarditis morbidity 100 %. Histologically we observed increased wall thickness with biventricular macrophage-rich mixed inflammatory infiltrates. All rats with a histologically severe myocarditis showed increased native T1 and decreased post-contrast T1 of the myocardium.

ConclusionsThe assessment of native T1 and post-contrast T1 allows accurate differentiation between healthy myocardium and myocardium with inflammation and also between the acute and chronic phases of the disease.

KeywordsT1 mapping Autoimmune myocarditis Lewis rat Dilated cardiomyopathy Cardiovascular magnetic resonance AbbreviationsBWbody weight

CMRCardiovascular Magnetic Resonance

CVFCollagen volume fraction

EAMexperimental autoimmune myocarditis

ECVExtracellular Volume Fraction

EFejection fraction

HEhematoxylin and eosin

HWheart weight

LGELate Gadolinium Enhancement

LVLeft ventricular

SALLISmall Animal Lock-Locker Inversion Recovery

SPFspecific pathogen free

SVstroke volume

TEecho time

TRrepetition time

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Author: Sarah Jeuthe - Katharina Wassilew - Darach O h-Ici - Tiago Ferreira da Silva - Frédéric Münch - Felix Berger - Titus Ku


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