Applying 1H NMR Spectroscopy to Detect Changes in the Urinary Metabolite Levels of Chinese Half-Pipe Snowboarders after Different ExercisesReportar como inadecuado




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Journal of Analytical Methods in Chemistry - Volume 2015 2015, Article ID 315217, 9 pages -

Research Article

College of P.E and Sports, Beijing Normal University, No. 19 Xinjiekouwai Street, Haidian District, Beijing 100875, China

Center of Analysis and Test, Beijing Normal University, No. 19 Xinjiekouwai Street, Haidian District, Beijing 100875, China

School of Chemical Engineering and Technology, Tianjin University, 92 Weijin Road, Nankai District, Tianjin 300072, China

Received 11 March 2015; Revised 10 May 2015; Accepted 11 May 2015

Academic Editor: Gowda A. Nagana Gowda

Copyright © 2015 Fuqiu Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Monitoring physical training is important for the health and performance of athletes, and real-time assessment of fatigue is crucial to improve training efficiency. The relationship between key biomarkers and exercise has been reported. The aim of this study was to determine the effects of different levels of training exercises on the urine metabolome.

H NMR-based metabolomics analysis was performed on urine samples from half-pipe snowboarders, and spectral profiles were subjected to PCA and PLS-DA. Our results show that metabolic profiles varied during different stages of exercises. Lactate, alanine, trimethylamine, malonate, taurine, and glycine levels decreased while TMAO and phenylalanine levels increased in the stage with higher amount and intensity of exercise. Although the amount of exercise was reduced in subsequent stage, no significant variations of metabolic profile were found. Metabolic changes induced by training level were analyzed with related metabolic pathway. Studying metabolome changes can provide a better understanding of the physiology of athletes and could aid in adjusting training.





Autor: Fuqiu Wang, Jiao Han, Qing He, Zhufeng Geng, Zhiwei Deng, and Decai Qiao

Fuente: https://www.hindawi.com/



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