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1 LIPhy - Laboratoire Interdisciplinaire de Physique Saint Martin d’Hères 2 LOB - Laboratoire d-optique et biosciences

Abstract : We characterize experimentally the influence of sample structure and beam focusing on signal level in third-harmonic generation THG microscopy. In the case of a homogeneous spherical sample, the dependence of the signal on the size of the sphere can be controlled by modifying the Rayleigh length of the excitation beam. More generally, the influence of excitation focusing on the signal depends on sample geometry, allowing one to highlight certain structures within a complex system. We illustrate this point by focusing-based contrast modulation in THG images of Drosophila embryos.

Keywords : optical harmonic generation optical microscopy optical focusing photoexcitation optical modulation biological techniques

Autor: Delphine Débarre - Willy Supatto - Emmanuel Beaurepaire -



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