Velocimetric third-harmonic generation microscopy: micrometer-scale quantification of morphogenetic movements in unstained embryosReportar como inadecuado




Velocimetric third-harmonic generation microscopy: micrometer-scale quantification of morphogenetic movements in unstained embryos - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

1 LIPhy - Laboratoire Interdisciplinaire de Physique Saint Martin d’Hères 2 LOB - Laboratoire d-optique et biosciences 3 RNSC - Réseau National des Systèmes Complexes 4 INRA

Abstract : We demonstrate the association of third-harmonic generation THG microscopy and particle image velocimetry PIV analysis as a novel functional imaging technique for automated micrometer-scale characterization of morphogenetic movements in developing embryos. Using a combined two-photon-excited fluorescence and THG microscope, we characterize the optical properties of Drosophila embryos and show that sustained THG imaging does not perturb sensitive developmental dynamics. Velocimetric THG imaging provides a quantitative description of the dynamics of internal structures in unstained wild-type and mutant embryos.

Keywords : microscopy nonlinear optics optical harmonic generation multiphoton processes optical microscopy biophysics turbidity dispersive media absorbing media biology





Autor: Delphine Débarre - Willy Supatto - Emmanuel Farge - Bruno Moulia - Marie-Claire Schanne-Klein - Emmanuel Beaurepaire -

Fuente: https://hal.archives-ouvertes.fr/



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