Purification of an Exopolygalacturonase from Penicillium viridicatum RFC3 Produced in Submerged FermentationReportar como inadecuado




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International Journal of MicrobiologyVolume 2009 2009, Article ID 631942, 8 pages

Research ArticleLaboratory of Biochemistry and Applied Microbiology, Ibilce, Universidade Estadual Paulista Unesp, Rua Cristovao Colombo, 2265, Jd. Nazareth, São José do Rio Preto, SP, CEP 15054-000, Brazil

Received 11 August 2009; Revised 1 October 2009; Accepted 3 November 2009

Academic Editor: Michael A. Cotta

Copyright © 2009 Eleni Gomes et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

An exo-PG obtained from Penicillium viridicatum in submerged fermentation was purified to homogeneity. The apparent molecular weight of the enzyme was 92 kDa, optimum pH and temperature for activity were pH 5 and 50– C. The exo-PG showed a profile of an exo-polygalacturonase, releasing galacturonic acid by hydrolysis of pectin with a high degree of esterification D.E

Ions enhanced the stability of enzyme and its activity by 30%. The was 1.30 in absence of and 1.16 mg in presence of this ion. In relation to the the presence of this ion increased from 1.76 to 2.07  mol .





Autor: Eleni Gomes, Rodrigo Simões Ribeiro Leite, Roberto da Silva, and Dênis Silva

Fuente: https://www.hindawi.com/



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