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International Journal of Inflammation - Volume 2017 2017, Article ID 5968618, 8 pages -

Research ArticleBrighton Studies in Tissue-Mimicry and Aided Regeneration BrightSTAR, Brighton Centre for Regenerative Medicine, School of Pharmacy and Biomolecular Sciences, University of Brighton, Lewes Road, Brighton BN2 4GJ, UK

Correspondence should be addressed to A. L. Guildford

Received 10 January 2017; Revised 26 April 2017; Accepted 7 May 2017; Published 6 June 2017

Academic Editor: Han J. Moshage

Copyright © 2017 T. R. Elliott and A. L. Guildford. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Pernicious anaemia PA is an autoimmune condition where antibodies target intrinsic factor and parietal cells, reducing the patient’s ability to absorb cobalamin promoting atrophic gastritis. Treatment guidelines are based on excretion data of hydroxocobalamin from healthy individuals obtained 50 years ago. This manuscript describes the use of phorbol 12-myristate 13-acetate PMA to stimulate low grade inflammation in an epithelial colorectal cell line to assess the efficacy of methylcobalamin and hydroxocobalamin. Nitric oxide increased significantly in cells exposed to higher doses of PMA 100 ng-ml, 150 ng-ml, and 200 ng-ml accompanied by a loss of the characteristic cobblestone morphology with no negative effect on cell activity or viability. A significant reduction in nitric oxide production was associated with the addition of 200 pg-ml hydroxocobalamin, alongside a return to the characteristic cobblestone morphology. This study highlights the use of PMA to promote low grade inflammation in human cell lines to model gastric inflammation associated with autoimmunity; furthermore it raises questions regarding the concentration of cobalamin administered clinically to restore cell functionality, feasibly allowing the patient to receive reduced quantity of the vitamin more regularly, providing the patient with levels which are akin to dietary intake.

Autor: T. R. Elliott and A. L. Guildford



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