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Mediators of Inflammation - Volume 2014 2014, Article ID 959471, 10 pages -

Research Article

Department of Oral Biology and Pathology, School of Dental Medicine, Stony Brook University, Stony Brook, NY 11794, USA

Department of Diagnosis and Surgery, School of Dentistry at Araraquara, UNESP, 14801-903 Araraquara, SP, Brazil

Department of Chemistry, Stony Brook University, Stony Brook, NY 11794-3400, USA

Received 28 February 2014; Revised 1 May 2014; Accepted 19 May 2014; Published 29 June 2014

Academic Editor: Fabio S. Lira

Copyright © 2014 Muna S. Elburki et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Tetracycline-based matrix metalloproteinase- MMP- inhibitors are currently approved for two inflammatory diseases, periodontitis and rosacea. The current study addresses the therapeutic potential of a novel pleiotropic MMP-inhibitor not based on an antibiotic. To induce experimental periodontitis, endotoxin LPS was repeatedly injected into the gingiva of rats on one side of the maxilla; the contralateral control side received saline injections. Two groups of rats were treated by daily oral intubation with a chemically modified curcumin, CMC 2.24, for two weeks; the control groups received vehicle alone. After sacrifice, gingiva, blood, and maxilla were collected, the jaws were defleshed, and periodontal alveolar bone loss was quantified morphometrically and by μ-CT scan. The gingivae were pooled per experimental group, extracted, and analyzed for MMPs gelatin zymography; western blot and for cytokines e.g., IL-1β; ELISA; serum and plasma samples were analyzed for cytokines and MMP-8. The LPS-induced pathologically excessive bone loss was reduced to normal levels based on either morphometric or μ-CT analysis. A similar response was seen for MMPs and cytokines in the gingiva and blood. This initial study, on a novel triketonic zinc-binding CMC, indicates potential efficacy on inflammatory mediators and alveolar bone loss in experimental periodontitis and warrants future therapeutic and pharmacokinetic investigations.





Autor: Muna S. Elburki, Carlos Rossa, Morgana R. Guimaraes, Mark Goodenough, Hsi-Ming Lee, Fabiana A. Curylofo, Yu Zhang, Francis

Fuente: https://www.hindawi.com/



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