Only one of the two type VI secretion systems encoded in the Salmonella enterica serotype Dublin genome is involved in colonization of the avian and murine hostsReportar como inadecuado




Only one of the two type VI secretion systems encoded in the Salmonella enterica serotype Dublin genome is involved in colonization of the avian and murine hosts - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

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Abstract : The type VI secretion system T6SS is a virulence factor for many Gram-negative bacteria. Salmonella genus harbors five phylogenetically distinct T6SS loci encoded in Salmonella Pathogenicity Islands SPIs SPI-6, SPI-19, SPI-20, SPI-21 and SPI-22, which are differentially distributed among serotypes. The T6SSs encoded in SPI-6 and SPI-19 contribute to pathogenesis of serotypes Typhimurium and Gallinarum in mice and chickens, respectively. Salmonella Dublin is a pathogen restricted to cattle where it causes a systemic disease. Also, it can colonize other hosts such as chickens and mice, which can act as reservoirs of this serotype. Salmonella Dublin harbors the genes for both T6SSSPI-6 and T6SSSPI-19. This study has determined the contribution of T6SSSPI-6 and T6SSSPI-19 to host-colonization by Salmonella Dublin using avian and murine models of infection. Competitive index experiments showed that, a mutant strain lacking both T6SSs ∆T6SSSPI-6-∆T6SSSPI-19 presents a strong colonization defect in cecum of chickens, similar to the defect observed for the ∆T6SSSPI-6 mutant, suggesting that this serotype requires a functional T6SSSPI-6 for efficient colonization of the avian gastrointestinal tract. Colonization of mice was also defective, although to a lesser extent than in chickens. In contrast, the T6SSSPI-19 was not necessary for colonization of either chickens or mice. Transfer of T6SSSPI-6, but not T6SSSPI-19, restored the ability of the double mutant to colonize both animal hosts. Our data indicate that Salmonella Dublin requires only the T6SSSPI-6 for efficient colonization of mice and chickens, and that the T6SSSPI-6 and T6SSSPI-19 are not functionally redundant.





Autor: David Pezoa Carlos J Blondel Cecilia A Silva Hee-Jeong Yang Helene Andrews-Polymenis Carlos A Santiviago Inés Contreras -

Fuente: https://hal.archives-ouvertes.fr/



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